切创后细胞自噬及炎症因子的表达与损伤时间的关系

Expression of autophagy and inflammation cytokines in mice wound skin and their relationship with the time of wound

目的观察小鼠皮肤切创后不同时间细胞自噬与炎症细胞因子的变化,同时探讨这些变化与损伤时间之间的关系。方法采用RT-PCR及West-blot方法检测小鼠皮肤切创组织在不同时间Beclin-1、LC-3、IL-1α、IL-1β、MCP-1的基因表达及蛋白表达。结果皮肤切创后早期IL-1α、IL-1β基因的表达及蛋白表达水平有所升高,12小时达到最高值,而后呈下降的趋势;MCP-1的表达在损伤后6h开始升高,48h达到最高值,72h有所下降,而Beclin-1、LC3基因及蛋白表达在组织损伤后6h开始升高,24h到达到最高值而后下降。结论小鼠皮肤组织切创后细胞自噬的启动及自噬的最高值明显晚于炎性细胞因子的启动及炎症因子表达的最高值,实验发现当细胞自噬水平达到最高时,炎症因子的水平则开始表现为下降的趋势。这可能是随着切创组织中炎症因子释放的增多激活了细胞的自噬,而当自噬水平达到一定的程度又抑制过度的炎症反应。自噬与炎症因子的表达随着小鼠皮肤创面损伤后不同时间呈现出一定的变化规律,这为组织损伤后损伤的时间推断提供一定的参考。

Objective To observe the performances of autophagy and inflammatory cytokines in wounded mice skin at different time, and to explore the relationship between these performances and the duration of wound in the mice. Methods RT-PCR and West-blot methods were used to detect the genetic expression and protein expression of Beclin-1, LC-3, IL-1α, IL-1β, and MCP-1 in wounded skin of the mice. Results After the mice＇s skin was wounded, the level of IL-1α and IL-1β genetic and protein expression increased early, the peak was found after 12 hours, and then decreased; the expression of MCP-1 began to increase after 6 hours and it reached peak after 48 hours, then decreased; while the genetic and protein expression of LC3 and beclin-1 began to increase after 6 hours, and the peak was seen after 24hours, then decreased too. Conclusion After the mice＇s skin was wounded, the starting and the peak of the expression of autophagy came later than those of inflammatory cytokines, it is found the level of the expression of inflammatory cytokines was showing a downward trend when the expression of autophagy reached the peak. This performance may be caused by increasing inflammatory cytokines in the tissue of the incised wound, which activated the autophagy, and when the level of autophagy reached a certain degree, it could suppress the excessive inflammatory reaction. So the autophagy and the inflammatory cytokines interact regularly after the mice＇s skin was wounded, and such interaction offers us a reference to infer the injury time after injury.