HPLC-DAD法同时测定糖痹康中6种主要活性成分

Simultaneous determination of 6 main active components in Tang-Bikang by HPLC-DAD

目的研究糖痹康颗粒6种活性成分的含量同时测定的方法。方法采用HPLC-DAD法测定糖痹康颗粒中6种活性成分的含量,C18色谱柱（150 mm,4.6 mm,5μm）,0.1%甲酸与水-0.1%甲酸与乙腈（18%-60%）梯度洗脱;检测λ为多波长检测分别是：196、201、202、204、276、228 nm;用标准曲线法测定。结果 6种活性成分在5-600μg/m L范围内线性关系良好,回归方程分别为：芍药苷：Y=227 988X＋182,R2=0.999 1;毛蕊异黄酮葡萄糖苷：Y=417 929 X-213,R2=0.999 7;特女贞苷：Y=199 569X-312,R2=0.998 3;迷迭香酸：Y=364 936X＋152,R2=0.998 1;黄芩苷：Y=64 131 X＋362,R2=0.999 4;小檗碱：Y=87 882 X＋111,R2=0.999 5。6种活性成分的加样回收率分别为：芍药苷：101.44%（RSD=0.48%）;毛蕊异黄酮葡萄糖苷：99.99%（RSD=0.43%）;特女贞苷102.07%（RSD=0.57%）;迷迭香酸：98.38%（RSD=0.46%）;黄芩苷：102.71%（RSD=0.485%）;小檗碱：99.54%（RSD=0.77%）。结论所建立含量测定方法简便、快捷、可靠,可用于糖痹康颗粒的质量控制。

Objective To study a method for simultaneous determination of 6 active components in Tang-Bikang granules. Methods Determination of 6 active ingredients of Tang-Bikang granules by HPLC-DAD method,C18column（150 mm,4. 6 mm,5 m）,0. 1% formic acid and water-0. 1% formic acid and acetonitrile（18%-60%） gradient elution;The detection wavelength is multi wavelength detection,they are：196,201,202,204,276,228 nm;Standard curve method was used. Results The 6 active ingredients in 5 μg·m L-600μg·m L shows a good linearity,and the regression equations respectively are：Peoniflorin：Y = 227 988 X ＋ 182,R2= 0. 999 1;Campanulin：Y = 417 929X-213,R2=0. 999 7;Specnuezhenide：Y = 199 569X-312,R2= 0. 998 3;Rosmarinci acid：Y = 364 936 X ＋ 152,R2= 0. 998 1;Ba-icalin：Y = 64 131 X ＋ 362,R2= 0. 999 4;Berberine：Y = 87 882 X ＋ 111,R2= 0. 999 5. The recoveries of the 6 active components are：Peoniflorin：101. 44%（ RSD 0. 48%）;Campanulin：99. 99%（ RSD 0. 43%）;Specnuezhenide：102.07%（ RSD 0. 57%）;Rosmarinci acid：98. 38%（ RSD 0. 46%）;Baicalin：102. 71%（ RSD 0. 485）;Berberine：99.54%（ RSD 0. 77%）. Conclusion The establishment of the content determination method is simple,fast and reliable,and it can be used to control the quality of Tang-Bikang particles.