抑癌基因RUNX3 rs760805位点T/A多态性与萎缩性胃炎的关系

The Relationship between Polymorphism of Tumor Suppressor Gene RUNX3 rs760805 T/A and the Morbidity of Atrophic Gastritis

目的探讨RUNX3基因多态性与胃癌的癌前病变——萎缩性胃炎（CGA）的关系。方法通过聚合酶链反应-限制性酶片段长度多态性（PCR-RFLP）对来自Hardy—Weinberg平衡群体的80例CGA和80例浅表性胃炎（CSG）患者RUNX3基因rs760805位点的基因型和等位基因频率进行分析。RUNX3基因rs760805位点的基因型和基因频率突变情况用x^2检验方法分析。通过非条件Logistic回归模型计算的风险值（OR）对rs760805位点各基因型与CGA发生风险度的关系进行分析。结果CGA组RUNX3基因rs760805位点TT、TA和AA基因型的频率分别占20．0％、42．5％和37．5％，CSG组TT、TA和AA基因型的频率分别为35．0％、40．O％和25．0％，差异有统计学意义（P〈0．05）。CGA组RUNX3基因rs760805住点等位基因A频率（58．75％）显著高于CSG组（45．00％）（P〈0．05）。与RUNX3基因rs760805基因位点TT基因型相比，TA基因型、AA基因型和TA＋AA基因型发生CGA的风险值分别为1．313（95％Ch0．940～1．833）、1．591（95％Ch1．060—2．389）和1．420（95％Ch1．051—1．918）．P值分别为0．059、0．011和0．017。结论与RUNX3基因rs760805位点TT基因型相比，AA和TA＋AA基因型显著增加了CGA发生的风险，与CGA的发生有一定的相关性。

Objective To investigate the relationship between RUNX3 gene polymorphism and chronic gastric atrophy （CGA）. Methods 80 CGA and 80 superficial gastritis （CSG） patients came from Hardy-Weinberg＇ s equilibrium. We used polymerase chain reaction-restriction fragment length polymorphism （PCR-RFLP） to analyze the genotypes frequencies and alleles frequencies of RUNX3 rs760805. The differences between genotypes frequencies and alleles frequencies of RUNX3 rs760805 sites were analyzed by using X 2 test. The odds ratio （OR） of various genotypes were calculated by unconditional logistic regression model to assess the relationship between the rs760805 genotypes and the risk of CGA. Results The genoty-pe frequencies of RUNX3 rs760805 TT, TA and AA in CGA were 20.0%, 42.5% and 37.5% respectively, and the frequencies of RUNX3 rs760805 TT, TA and AA in CSG were 35.0%, 40.0% and 25.0% respectively; there was a statistical difference between CGA and CSG （P〈0.05）. The frequency of RUNX3 gene rs760805 allele A in CGA （58.75%） was higher than that in CSG （45.00%） （P〈0.05） significantly. The OR of genotype TA, AA and TA＋AA is 1.313 （95% CI： 0.940 - 1.833）, 1.591 （95% CI： 1.060 - 2.389） and 1.420 （95% CI： 1.051 - 1.918） compared with TT genotype respectively. Conclusion RUNX3 rs760805 genotype AA and TA＋AA increased the risk of CGA significantly and it may be associated with the pathogenesis of CGA.