摘要
Gene Editing refers to the process of making targeted modi- fications to the genome, such as the deletion, insertion or replacement of targeted DNA sequences （Pan et al., 2011）. Gene editing technologies fundamentally improve our ap- proaches to biological basic research, gene therapy, genetic improvement, etc. In the field of stem cells, gene editing can be used to modulate cell fate, such as generation of in- duced pluripotent stem cells （iPSCs） （Ding et al., 2015）, cellular differentiation and transdifferentiation （Zhang et al., 2014）. In gene therapy, gene editing can be used to con＇ect disease mutations in patient-derived cells, which holds great promise to cure related diseases in the future （Liu et al., 2012; Liu et al., 2014）.
Gene Editing refers to the process of making targeted modifications to the genome,such as the deletion,insertion or replacement of targeted DNA sequences(Pan et al.,2011).Gene editing technologies fundamentally improve our approaches to biological basic research,gene therapy,genetic improvement,etc.In the field of stem cells,gene editing can be used to modulate cell fate,such as generation of induced pluripotent stem cells(i PSCs)(Ding et al.,2015),
