虾夷扇贝生殖腺酶解物-核糖美拉德反应产物抗氧化特性研究

Antioxidant activity of Maillard reaction products from system of ribose and hydrolyastes from scallop( Patinopecten yessoensis) gonad

本文选用碱性蛋白酶、中性蛋白酶、风味蛋白酶及木瓜蛋白酶制备虾夷扇贝雌性生殖腺酶解物,再与核糖进行美拉德反应获得虾夷扇贝雌性生殖腺酶解物-核糖美拉德反应产物。采用DPPH自由基和亚油酸过氧化体系,评价美拉德产物的抗氧化能力。结果显示,碱性蛋白酶酶解效果较好,在酶解3 h时其水解度及TCA可溶性寡肽得率最高,分别达24.16%和20.79%。以DPPH自由基清除活性及亚油酸过氧化抑制活性为指标,确定了pH7条件下制备的虾夷扇贝雌性生殖腺酶解物-核糖美拉德产物抗氧化能力相对较强。通过比较半抑制浓度(IC50),美拉德产物的抗氧化能力较酶解物及核糖本身均显著提高(p<0.05),但弱于抗氧化剂2,6-二叔丁基-4-甲基苯酚(BHT)。以上研究结果说明,虾夷扇贝生殖腺酶解物-核糖美拉德反应产物具有一定的抗氧化能力,具有潜在的开发价值。

In this paper, the hydrolysates from scallop（ Patinopecten yessoensis）female gonad were prepared by using alcalase, neutrase,flavourzyme and papain.Maillard reaction products were further obtained from the system of ribose and hydrolysates from scallop（Patinopecten yessoensis）female gonad.The antioxidant capacities of maillard reaction products were evaluated by DPPH radical scavenging assay and liuoleic acid peroxidation assay.The results showed that alcalase had the better hydrolysis effect than the other enzymes.The hydrolysis degree and TCA soluble peptide yield reached 24.16% and 20.79% ,respectively, after hydrolysis for 3 h. The Maillard reaction products of scallop female gonad hydrolysates- ribose showed the stronger antioxidant abilities at pH7 of by using DPPH radical-scavenging activity and anti-linoleic acid peroxidation capacity as the indicators.By comparing the half inhibition concentration （IC50）, the antioxidant capacity of Maillard reaction products was significantly enhanced as comparison with the hydrolysates and ribose （p 〈 0.05 ）, but weaker than butylated hydroxytoluene （BHT） .The results suggest that the Maillard reaction products of scallop female gonad hydrolysates-ribose possess antioxidant capacity, having potential value for exploitation.