摘要
本研究通过玛卡的defensin基因序列,设计了玛卡的特异性引物,建立了玛卡及其制品中玛卡源性成分检测的一种新方法。通过物种特异性测试,所设计的引物能够特异性扩增玛卡基因,并且,DNA浓度的检测灵敏度达到0.1 ng/μL以上,重量灵敏度达到0.1%以上。并结合芜菁源性成分基因检测方法对市场随机抽取的21份玛卡及其制品进行检测,21份样品均检出了玛卡源性成分而未检出芜菁源性成分。本研究所建立的PCR检测方法快速,准确,高效,适用于市场上玛卡及其制品真伪的快速鉴别。
According to the defensin gene sequences of Lepidium meyenii, a pair of specific primers of Lepidium meyenii was designed and a novel method for detection of Lepidium meyenii- origin genes was established. Based on the species- specificity and sensitivity testing,the designed Lepidium meyenii primer was of good species-specific and the novel method was of high sensitivity, with 0.1 ng/mL of DNA sensitivity and 0.1% of weight sensitivity, respectively. Using this method combining with the detection of rappini-origin genes, 21 samples of Lepidium meyenii and its manufactured goods from the market were randomly measured.The results showed that all the samples contained the Lepidium meyenii-origin genes,but not the rappini- origin genes.The PCR method established in this study was rapid, accurate, efficient and suitable for the identification of Lepidium meyenii and its manufactured goods from the market.
作者
刘冬虹
王德莲
郭燕华
聂炎炎
吴环
张慧
杨静
陈遂
曾国权
陈景亮
LIU Dong- hong WANG De- Lian GUO Yan- Hua NIE Yan- yan WU Huan ZHANG Hui YANG Jing CHEN Sui ZENG Guo- quan CHEN Jing- liang(Guangzhou Quality Supervision and Testing Institute, Guangzhou 511447, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第1期319-321,333,共4页
Science and Technology of Food Industry
基金
广东省质量技术监督局科技项目(2016CZ11)
关键词
玛卡
PCR
鉴别
maca
Lepidium meyenii
polymerase chain reaction
identification