摘要
目的:探究紫山药提取物(Purple yam extract,PYE)抗氧化活性与延长寿命作用。方法:将2 d龄雌性果蝇随机分成4组,在其培养基中分别添加不同浓度(0、0.5、2.0、4.0 mg/m L)PYE。研究PYE对果蝇寿命,超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和丙二醛(MDA)等抗氧化酶活力及抗氧化相关基因表达的影响;通过急性实验研究PYE对果蝇氧化应激损伤的影响。结果:与对照组相比,2.0、4.0 mg/m L PYE能显著延长果蝇的寿命(p<0.05);2.0、4.0 mg/mL PYE能显著提高SOD、CAT活力(p<0.05),显著降低MDA含量(p<0.05);2.0、4.0 mg/m L PYE能显著上调SOD、CAT mRNA表达水平(p<0.05),Methuselah(MTH)mRNA表达水平显著下调(p<0.05);急性实验中PYE组果蝇的寿命明显延长。结论:PYE在体内具有较强的抗氧化作用,并可能通过上调抗氧化基因的表达实现延长果蝇寿命的作用。
Objective : To explore the effect of purple yam extract ( PYE ) on antioxidant function and lifespan in Drosophila melanogaster.Methods:2 day-old female Drosophila melanogaster were divided into four groups randomly, and supplemented with different doses(0,0.5,2.0,4.0 mg/mL)of PYE.The life span, activities of antioxidant enzymes and their encoding genes expressions were detected.Acute experiment were performed to investigate the effects of PYE on oxidative stress injury.Results: Compared with the control group, PYE at 2.0 and 4.0 mg/mL could significantly prolong the lifespan of Drosophila melanogaster, and increase the activities of SOD and CAT and decrease MDA level(p 〈 0.05 ).The mRNA expressions of SOD and CAT with PYE at 2.0 and 4.0 mg/mL were up-regulated significantly (p 〈 0.05 ) , while the Methuselah (MTH)mRNA level was significantly down - regulated ( p 〈 0.05 ) . The lifespan of Drosophila melanogaster treated with PYE was extended significantly in the aeute tests. Conclusion: PYE has significant antioxidant capacity in vivo and can prolong the lifespan of Drosophila melanogaster by up-regulating the expression of antioxidant genes.
作者
王彦平
袁贵英
曹娅
谢克英
孙瑞琳
汤高奇
WANG Yan-ping YUAN Gui-ying CAO Ya XIE Ke-ying SUN Rui-lin TANG Gao-qi(Henan Vocational College of Agriculture, Zhengzhou 451450, China)
出处
《食品工业科技》
CAS
CSCD
北大核心
2017年第1期360-363,374,共5页
Science and Technology of Food Industry
基金
郑州市普通科技攻关项目(153PKJGG424)
2015年度河南省高等学校优秀教学团队建设(河南农业职业学院食品营养与检测专业)
2014年度河南省高等学校"专业综合改革试点"项目(河南农业职业学院食品营养与检测专业)
关键词
紫山药提取物
果蝇
抗氧化
寿命
purple yam extract
Drosophila melanogaster
antioxidation
lifespan