丹参提取液对镉中毒大鼠肾损伤的抑制作用

Effects of water extract of salvia miltiorrhiza aganst renal injury on rats exposed to cadmium

目的探讨丹参水提液对染镉大鼠肾损伤的抑制作用机制。方法将雌雄各20只的Wistar大鼠随机分为空白对照组8只，模型组32只，常规饲养，空白对照组腹腔注射生理盐水5ml·kg^-1·d^-1，模型组以2．8mg·kg^-1·d^-1氯化镉溶液腹腔注射。待造模成功后，将模型组分为给药组（丹参水提液高剂量2．7g·kg^-1·d^-1、丹参水提液低剂量1．35g·kg^-1·d^-1）、阴性对照组、阳性对照组。在实验进行到4周后，给药组采用丹参水提液进行灌胃，阳性对照组采用亚硒酸钠溶液0．05mg·kg^-1·d^-1灌胃，阴性对照组、空白对照组给予同体积生理盐水灌胃2．7ml·kg^-1·d^-1各组干预治疗10周，于实验终末收集血清，分别检测血清血清超氧化物歧化酶（SOD）、丙二醛（MDA）、和总抗氧化能力（T-AOC）等指标。结果丹参水提液可使染镉大鼠体重增长较快、血清SOD[（241．0±2．8）U／ml比（219．6±4．6）U／ml，P〈0．05]明显升高、血清MDA[（7．2±0．3）μmol／L比（11．9±0．9）μmol／L，P〈0．01]较大降低、能够增强血清T—AOC[（8．7±1．5）U／ml比（4．6±2．0）U／ml，P〈0．05]。结论丹参水提液具有明显的抗氧化损伤的作用，在降低。肾小管上皮细胞肿胀，改善肾功能方面疗效显著。

Objective To investigate the effects of water extract of salvia miltiorrhiza aganst renal injury on rats exposed to cadmium. Methods Twenty female Wistar rats were randomly divided into blank control group （8）, 32 rats in model group, conventional breeding, the blank control group received intraperitoneal injection of saline 5 ml·kg^-1·d^-1, model group with 2. 8 mg·kg^-1·d^-1 of cadmium chloride solution by intraperitoneal injection. After the success in modeling, the model group was divided into treatment group （ salvia extract high dose 2. 7 g·kg^-1·d^-1, low water extract of salvia a dose of 1.35 g·kg^-1·d^-1）, negative control group, positive control group. After 4 weeks to experiment in the drug group, the water extract of salvia miltiorrhiza by intragastric administration, the positive control group using sodium selenite solution 0. 05 mg·kg^-1·d^-1 gavage, negative control group, the control group was given the same physical volume of saline water gastric 2. 7 ml·kg^-1·d^-1, respectively. Each group intervention for 10 weeks. At the end of the experiment, serum was collected at, were used to detect the serum oxide dismutase （ SOD ）, malondialdehyde （ MDA ）, serum total antioxidant capacity （ T-AOC ） . Results Danshen aqueous extract can make dye CD rats body weight growth faster, the serum SOD activity decreased significantly[ （241.0 ± 2. 8） U/ml vs （219.6 ±4. 6） U/ml, P 〈 0. 05 ], serum MDA increased greatly [ （ 7.2 ± 0. 3 ） μmol/L vs （ 11.9 ± 0. 9）μmol/L, P 〈 0. 01 ], to enhance the serum total antioxidant capacity [ （8.7 ±1.5 ） U/ml vs （4. 6 ± 2. 0） U/ml, P 〈 0. 05 ]. Conclusion Danshen aqueous extract has obvious antioxidant injury, in reducing swelling of renal tubular epithelial cells, improve renal function effect significantly.