摘要
目的揭示抗内皮细胞抗体(antiendothelial cell antibody,AECA)在突发性感音神经性聋(sudden sensorineural hearing loss,SSNHL)发病中的相关作用机制。方法本研究中我们进一步确定AECA和临床上SSNHL病例的一些临床特征关系;评估AECA在内皮细胞活化和凋亡中的作用。结果首先通过ELISA法测定67例SSNHL患者及50例对照组AECA水平。AECA促进肿瘤坏死因子α,E-选择素和细胞间黏附分子-1在人脐静脉内皮细胞中的表达。亚倍体细胞和膜联蛋白V细胞计数发现SSNHL-AECA+-Ig G组的阳性细胞数比SSNHL-AECA^--IgG组、正常对照组-IgG及内皮细胞生长培养基组显著升高。结论研究提示AECA可能对SSNHL发病起一定作用。
OBJECTIVE To clarify the role of antiendothelial cell antibody（AECA） in the etiology of sudden sensorineural hearing loss（SSNHL）.METHODS In present study,we further confirmed the relationship between AECA and clinical characteristics of SSNHL cases,and evaluated effects of AECA on epithelial cells activation and apoptosis.RESULTS The level of AECA in 67 patients with SSNHL and 50 control subjects was first determinated by ELISA assessment.AECA promoted expression of ICAM-1,E-selectin and TNF-α in HUVEC Cells.We found that both the number of hypoploid cell and annexin V positive cells in SSNHL-AECA~＋-IgG group were dramatically increased compared with SSNHL-AECA^--IgG,control-IgG and EMG group.CONCLUSION The results of the present study give us a novel insight that AECA may play a pivotal role in pathogesis of SSNHL.
出处
《中国耳鼻咽喉头颈外科》
CSCD
2016年第12期709-713,共5页
Chinese Archives of Otolaryngology-Head and Neck Surgery
基金
浦东新区科技发展基金创新资金(PKJ2013-Y24)
浦东新区卫生系统学科带头人培养计划项目(PWRd2015-06)
浦东新区"已病防变特色专病"建设项目(PDZYXK-2-2014006)联合资助
