摘要
目的探究注射用脑蛋白水解物对大鼠肾上腺嗜铬细胞瘤(PC12)细胞增殖与1-甲基-4-苯基吡啶(MPP^+)诱导的细胞氧化损伤的影响,为建立评价脑蛋白水解物活力检测方法提供科学依据。方法通过MPP^+损伤PC12细胞后,采用MTT法测定不同厂家、不同浓度的注射用脑蛋白水解物对PC12细胞的修复作用,通过测定其吸光度与正常细胞吸光度的比值来计算样品对PC12细胞损伤的修复率,以此作为注射用脑蛋白水解物活力考察的指标。同时还对注射用脑蛋白水解物对于MPP^+损伤的不同亚型的PC12细胞作用进行了研究。结果注射用脑蛋白水解物作用时间为48 h、浓度为60μg/ml时对MPP^+诱导的PC12细胞的氧化损伤具有较好保护作用,并且对低分化型的PC12细胞具有更明显的保护作用。结论注射用脑蛋白水解物可促进低分化型PC12细胞的增殖,并减轻MPP^+的损伤作用。研究建立的活力测定方法已经被国家标准采纳。
Objective To study the effects of cerebroprotein hydrolysate injection on the proliferation and MPP^＋ induced damages in rat pheochromocytoma cells（PC12）. Methods Cerebroprotein hydrolysate injection was used to protect PC12 cells from MPP^＋ induced damages. The bioactivity of cerebroprotein hydrolysate injection on different PC12 cell subtypes was determined by MTT assay, and the ratio of absorbance denoted its rehabilitation rate. Results With cerebroprotein hydrolysate injection 48 hours pre-treatment, the survival rate of MPP^＋ damaged PC12 cells was increased, and the optimal concentration was 60 μg/ml. Cerebroprotein hydrolysate injection reached better result for poorly differentiated PC12 cells. Conclusion Cerebroprotein hydrolysate injection protects against MPP^＋-induced reductions in PC12 cell viability and increased proliferation of PC12 cells. Our method has been adopted by Chinese Pharmacopoeia Commission（WS1-XH-017-2016）.
出处
《中国医药生物技术》
2016年第6期515-520,共6页
Chinese Medicinal Biotechnology
基金
北京协和医学院研究生创新基金(2015-1007-23)
