低频脉冲电磁场对高原冻伤血液循环恢复的影响

Effects of low frequency pulsed electromagnetic fields on restoration of plateau frostbite blood circulation

目的 :研究评估20 Hz、25 m T低频脉冲电磁场(low frequency pulsed electromagnetic fields,LFPEF)对高原冻伤伤口愈合过程中各组织层血液循环恢复情况的影响。方法:雄性SD大鼠24只,随机等分为3组:空白对照组(CG组)、高原冻伤组(PF组)和高原冻伤照射组(PF+LFPEF组)。PF组和PF+LFPEF组大鼠缺氧习服1周后,采用液氮冻伤的方法构建中度冻伤模型。采用低频脉冲电磁场发生器产生20 Hz、25 m T低频脉冲电磁场对PF+LFPEF组进行照射治疗。于造模后第4、7、10、13、16、19、22 d,采用超声微泡造影方法对各组大鼠冻伤伤口血液循环恢复情况进行观察检测,并计算对比各组冻伤部位微泡浓度。结果:在不同的时间点,PF+LFPEF组微泡浓度明显高于PF组,且在第22 d微泡浓度与CG组已无明显差别。结论:该低频脉冲电磁场能够有效促进高原冻伤血液循环的恢复和血管网络的形成。

ObjectiveTo evaluate the effects of low frequency pulsed electromagnetic fields （LFPEF） of 20 Hz and 25 mT on the restoration of plateau frostbite （PF） blood circulation during the wound healing. Methods Twenty-four male Sprague- Dawley rats were assigned to three equal groups： control, PF and PF＋LFPEF groups. The PF and PF＋LFPEF groups were acclimated to a hypoxic and low-pressure environment forlweek, and then the partial-thickness PF models were constructed with the liquid nitrogen freezing method. The LFPEF equipment generated the LFPEF of 20 Hz and 25 rat to expose and treat the rats of PF and PF＋LFPEF group. After operation on day 4, 7, 10, 13, 16, 19 and 22, the restoration situation of blood circulation of each group was observed with contrast ultrasonography. Finally, the values of mierobubble concentration were calculated and compared. Results On different time, the values of microbubble concentration of PF ＋LFPEF group were significantly higher than those of the PF group. In addition, the microbubble concentration difference between the PF＋LFPEF and PF group could not be found on the 22th day. Conclusion The LFPEF could efficiently accelerate the restoration of PF wound blood circulation and develop the capillary network.