TRIM22与IL-18的表达与宫颈癌的关系

EXPRESSION OF TRIM22 AND IL-18 LEVELS IN PATIENTS WTH CERVICAL CANCER

目的探讨三结构域家族蛋白22(Tripartite motif containing 22,TRIM22)与白细胞介素18(interleukin 18,IL-18)在伴有高危人乳头状瘤病毒(high risk human papilloma virus,HPV)感染的宫颈癌患者组织和血清中的表达,探讨高危HPV感染后宫颈癌的发病机制,为宫颈癌的早期诊断和治疗提供实验依据。方法分别采集临沂市妇女儿童医院27名伴有高危HPV感染的宫颈癌患者,27名高危HPV阳性但病理检查证实不是宫颈癌的患者,以及27名高危HPV阴性的健康女性的宫颈组织标本和血清标本,以免疫组织化学染色技术检测TRIM22在宫颈组织中的表达情况,用酶联免疫比色法检测血清中干扰素的水平。结果在伴有高危HPV感染的宫颈癌组、高危HPV阳性的非宫颈癌组、高危HPV阴性的非宫颈癌组三组人群中,TRIM22的阳性表达率分别为:33%、66%、85%;宫颈癌组与非宫颈癌的两组之间比较差异有显著性(P<0.05),在非宫颈癌的两组中,高危HPV阳性组与高危HPV阴性组之间比较有显著差异(P<0.05)。IL-18在三组研究对象血清中的水平分别为(84.16±17.41)、(43.48±12.19)、(33.53±7.14)pg/ml,宫颈癌组与非宫颈癌的两组之间比较差异有显著性(P<0.05),HPV阳性组与HPV阴性组之间比较有显著差异(P<0.05)。结论 IL-18的表达与HPV感染和癌症的发生正相关,而TRIM22的表达与高危HPV的感染和宫颈癌的发生负相关,提示固有免疫参与了高危HPV感染后宫颈癌的发生,IL-18可能作为宫颈癌早期诊断的生物学标志物。

Objective To analyze the expressions of tripartite motif containing 22 （TRIM22） and inter‐leukin 18 （IL‐18）in serum and tissues of patients with cervical cancer accompanied by high risk human pap‐illoma virus infection .To explore the pathogenesis of cervical cancer .Methods 81 patients’ tissues and se‐rum were enrolled in this study ,including 27 patients with high risk HPV infection and cervical cancer ,27 patients with high risk HPV infection but without cervical cancer and 27 patients without cervical cancer accompanying high risk HPV infection .The expressions of TRIM22 were measured by immunohistochem‐istry assay .The expression of IL‐18 were measured by enzyme linked immunoassorbent assay .Results The ex‐pression of TRIM22 in patients with cervical cancer and high risk HPV infection ,patients with high risk HPV infection and without cervical cancer ,patients without high risk HPV infection and cervical cancer were 33% 、66% 、85% respectively ,with statistically significant difference （ P 〈0 .05） .The expressions of IL‐18 in the three groups were （84 .16 ± 17 .41）、（43 .48± 12 .19）、（33 .53 ± 7 .14） pg/ml ,with statistically signifi‐cant difference（ P 〈0 .05） .Conclusion The expression of TRIM22 is negatively correlated with the infec‐tion of high risk HPV and cervical cancer .The expression of IL‐18 is positively correlated with the infec‐tion of high risk HPV and cervical cancer .Innate immunology is enrolled in the development of cervical cancer .IL‐18 could be regarded as a new biological marker in the early diagnosis of cervical cancer .