重度子痫前期胎盘组织中NFAT5表达与围生儿结局的相关性研究

Expression of NFAT5 in placenatal of severe preeclampsia and its relationship with perinatal outcome

目的:探讨核转录因子5(NFAT5)在重度子痫前期(sPE)胎盘中的表达及其与围生儿结局的相关性。方法:收集2015年1月至2016年1月在郑州大学第三附属医院行剖宫产分娩的sPE孕妇(试验组)60例。选取同期非PE行剖宫产术的孕妇60例作为对照组。免疫组化法检测胎盘组织中NFAT5表达;RT-PCR法检测胎盘组织中NFAT5 mRNA表达。对胎盘中NFAT5 mRNA水平与围生儿结局进行相关分析。结果:sPE组的新生儿体重、胎盘重量、Apgar评分及分娩孕周均低于对照组(P均<0.05)。两组胎盘中NFAT5蛋白多位于胎盘滋养细胞的胞核与胞质中,绒毛间质细胞内有少量表达。sPE组的NFAT5蛋白和mRNA表达水平均高于对照组,差异均有统计学意义(t=-40.434,P=0.001;t=-6.696,P=0.001)。sPE组的NFAT5 mRNA相对表达量与新生儿出生体重、胎盘重量、分娩孕周均呈负相关(r分别为-0.554,-0.698,-0.612,P均<0.05),与孕妇体重指数(BMI)呈正相关(r=0.630,P=0.012)。结论:sPE组胎盘组织中NFAT5表达水平升高,与围生儿不良结局有关。

Objective：To explore the expression of nuclear factor of activated T cells （NFAT5） in the placenta tissues of severe Preeclampsia（sPE） and the relationship with peri-natal outcome. Methods：Placental samples were collected from 60 normal full-term singleton pregnant women（ control group） and 60 singleton pregnant women with sPE who had elective cesarean section in the Third Affiliated Hospital of Zhengzhou University between Jan. 2015 and Jan. 2016. Immunohistochemistry assay was used to detect the expression and distribution of NFAT5 in placental tissues. RT-PCR was conducted to analyze the expression of NFAT5 mRNA in placental tissues. The relationship between the NFAT5 expression and perinatal outcome were analyzed. Results：（ 1 ） There were significant difference between two groups about neonatal weight,the placenta weight,Apgar score and delivery gestational age （P〈0. 05）. The expression of NFAT5 protein and mRNA in placenta of the sPE group were significant higher than that of the control group （t=-40. 434,P=0. 001;t=-6. 696,P=0. 001）. NFAT5 protein were almost expressed in the nucleus and cytoplasm of the syncytiotrophoblasts cells and little was expressed in villi mesenchymal cells. The expression of NFAT5 mRNA had a negative correlation withbirth weight,placental weight,gestational age at birth（r=-0. 554,-0. 698,-0. 612;P〈0. 05）. The expression level of NFAT5 had positive correlations with body mass index （ r=0 . 630 , P=0. 012）. Conclusions：The levels of NFAT5 in the placenta are associated with the severity of preeclampsia and are associated with poor perinatal outcome.