离子束介导小麦变异材料贮藏蛋白及农艺品质性状分析

Analysis of Storage Protein and Agronomic Traits of Mutanted Wheat by Ion-beam

离子束诱导技术是一项非常有效的致使植物突变的技术,为提高小麦的品质性状,采用离子束介导外源全基因组的方法,转化选育出一批有价值的小麦变异材料。本研究对这批小麦变异材料的农艺性状、品质性状、各贮藏蛋白组分进行分析。发现变异材料的株高、千粒重、叶面积、湿面筋、SDS沉淀值、蛋白含量与对照相比均有明显的变化,并达到显著差异水平,但有一些变异材料存在抗病性不好或蛋白含量高但是品质不好的问题,需进一步的改进。变异材料的各蛋白组分含量与对照相比均有明显的差异且蛋白含量、湿面筋含量都有明显增加的趋势。研究结果表明,离子束介导技术产生丰富的变异,而且变异情况与基因受体密切相关,需要对这批变异材料进行进一步的特定培育,从而选育出种质资源良好、蛋白含量高且质量好的品种。

The technology of ion-beam mediation is a very effective method resulting in plant mutation. In order to improve the quality characters of wheat, we adopt the method of ion beam mediated exogenous total DNA,transformed and bred a number of valuable wheat variation materials.Then we analyzed the agronomic traits,quality traits and storage protein of variants, found obvious changes on height,thousand grain weight, leaf area, wet gluten content, SDS sedimentation value and protein content compared the control, and these changes had reached significant difference level. However, there are still some problems on the material whose disease resistance were not well or protein content was high but had not better quality. So these materials need further improvement. The content of storage protein of the variant materials had obvious difference with the control, and the content of protein and wet gluten had obvious increase. These results show that the ion beam mediated process help to produce rich variation, and the variation has a close relation to the receptor gene.we need to cultivate this batch of variation materials, and then breed some variety with good germplasm resources, high protein content and better quality.