摘要
【目的】为提高昆虫病原线虫对韭菜迟眼蕈蚊Bradysia odoriphaga Yang et Zhang幼虫的防治效果,将昆虫病原线虫与环境友好型化学杀虫剂混用是一条有效途径。【方法】测定了噻虫嗪与6个昆虫病原线虫品系混用对韭菜迟眼蕈蚊的作用效果,以及温度和土壤含水量对作用效果的影响,并进行了田间验证。【结果】田间推荐浓度噻虫嗪(100 mg·L^(-1))对6种供试线虫存活无显著影响。处理后3 d,低浓度噻虫嗪(15 mg·L^(-1))分别与6品系线虫混合后处理韭菜迟眼蕈蚊幼虫,其死亡率明显高于线虫和噻虫嗪单用处理。小卷蛾斯氏线虫SF-SN+噻虫嗪、印度异小杆线虫LN2+噻虫嗪和小卷蛾斯氏线虫All+噻虫嗪3种组合发挥杀虫作用的最适温度范围分别为20~25℃、25~30℃和25~30℃,显著高于其他温度;最适土壤含水量范围为10%~18%,也显著高于其他湿度。大田条件下,施用后7 d,单用噻虫嗪、线虫+噻虫嗪组合处理对韭菜迟眼蕈蚊的防治效果显著高于单线虫,且以芫菁夜蛾斯氏线虫SF-SN+噻虫嗪的防治效果最高,达到93%以上。【结论】芫菁夜蛾斯氏线虫SF-SN品系与噻虫嗪组合联合防治韭菜迟眼蕈蚊效果最好。
[Objectives] To increase the effectiveness of controlling Bradysia odoriphaga with entomopathogenic nematodes, and to screen pesticides as synergists for entomopathogenenic nematodes. [Methods] The survival rate and infectivity of six entomopathogenic nematodes (Steinernema carpocapsae NC116, S. carpocapsae All, S. longicaudum X-7, S. feltiae SF-SN, Heterorhabditis bacteriophora H06 and H. indica LN2) were tested after dipping them in a commonly used pesticide (25%water dispersible granules of thiamethoxam). The effects of treatment with six nematodes and low concentrations of thiamethoxam (12 mg·L-1) on 3rd instar larvae were determined. Interactions between three kinds of nematodes (S. feltiae SF-SN, H. indica LN2 and S. carpocapsae All), and low concentrations of thiamethoxam (30 mg·L-1), were evaluated under various temperature and humidity conditions and further tested under greenhouse conditions. [Results] The recommended concentration of thiamethoxam (100 mg·L-1) had no effect on nematode survival. Mortality of B. odoriphaga treated with a combination of six nematodes and a low concentration thiamethoxam (12 mg·L-1) was higher after 3 d than that of larvae that had been treated with thiamethoxam and any one nematode. Mortality of B. odoriphaga treated with S. feltiae SF-SN+thiamethoxam, H. indica LN2+thiamethoxam and S. carpocapsae All+thiamethoxam was higher at 20-25℃, 25-30℃, and 25-30℃, respectively, than at other temperatures. The optimum substrate moisture level for these treatments was 10%-18%. The results of greenhouse trials show that a combination treatment (S. feltiae SF-SN+thiamethoxam) achieved〉93%better control than treatment with thiamethoxam (100 mg×a.i./L) alone after 7 d. [Conclusion] The S. feltiae SF-SN+thiamethoxam combination appears an effective way of controlling B. odoriphaga.
作者
武海斌
凌飞
宫庆涛
付丽
许永玉
孙瑞红
WUHai-Bin LINGFei GONGQing-Tao FULi XUYong-Yu SUNRui-Hong(Shandong Institute of Pomology, Tai'an 271000, China Shandong Rizhao International Ocean City Investment CO. LTD, Rizhao 276800, China College of Plant Protection, Shandong Agricultural University, Tai'an 271018, China)
出处
《应用昆虫学报》
CAS
CSCD
北大核心
2016年第6期1233-1241,共9页
Chinese Journal of Applied Entomology
基金
公益性行业(农业)科研专项(201303027)
山东省农业科学院青年科研基金(2015YQN30)
关键词
昆虫病原线虫
噻虫嗪
韭菜迟眼蕈蚊
混合使用
entomopathogens nematodes
thiamethoxam
Bradysia odoriphaga
combination