茶蜂花粉抗氧化活性与成分

Antioxidant Activity and Composition of Camellia Been Pollen

本实验研究了茶蜂花粉的抗氧化活性和功效成分。茶蜂花粉85%乙醇回流提取,经过石油醚、乙酸乙酯、正丁醇依次萃取,得到石油醚萃取物、乙酸乙酯萃取物、正丁醇萃取物以及萃余物4个部分,用D-半乳糖诱导衰老小鼠模型检测茶蜂花粉不同组分的抗氧化活性,采用多种色谱方法分离具有抗氧化活性的成分,利用核磁共振方法鉴定各单体的化学结构。结果表明:茶蜂花粉乙醇提取物、乙酸乙酯萃取物具有明显的抗氧化作用,乙酸乙酯萃取物经MCI柱层析,得到30%、50%、70%三个乙醇洗脱组分,其中30%乙醇洗脱组分具有显著抗氧化活性,从该洗脱部分得到三个单体化合物,经鉴定为:槲皮素、山奈素-3-O-芸香糖苷、山奈酚-3-O-(2″,3″,4″-O-三对羟基桂皮酰基)-β-D-吡喃葡萄糖苷,推测茶蜂花粉具有抗氧化活性的成分主要为黄酮类化合物。

This experiment was aimed to study the antioxidant activity and functional components of camellia bee pollen. Camellia bee pollen was powdered and extracted by 85% ethanol under reflux and then was extracted by petroleum e- ther, ethyl acetate and n-butyl alcohol. D-Galactose induced aging mice model was used to detect the antioxidant activity of different components of camellia bee pollen. Various Chromatographic methods were employed to separate the ingredi-ents with antioxidant activity. Nuclear magnetic resonance （NMR） was applied to identify the chemical structure of the monomers. The results showed that the camellia been pollen ethanol extracts and ethyl acetate extracts had obvious an-tioxidant effect. Ethyl acetate extracts by MCI column chromatography was isolated into 30% ,50% ,70% ethanol elution contents. 30% Ethanol elution had obvious antioxidant activity. Three monomer compounds were separated and identified from the elution part ：quercetin, kaempferide-3-O-rutinoside and kaempferot-3-O-（2＂,3＂,4＂-O-hydroxyl cinnamon acyl）-β-D-glucopyranoside. Therefore,flavonoids were the main functional components in camellia bee pollen for the antioxidant activity.