沙棘Vp对辐射损伤小鼠的保护及免疫功能作用研究

Protective and Immunoregulation Effects of Sea Buckthorn Vp on Mice Injured by X-ray Radiation

本文探讨了沙棘Vp对X射线辐射损伤小鼠的保护作用。将150只昆明种小白鼠分为正常对照组、阳性对照组、辐射对照组、沙棘Vp高、中、低剂量组,雌雄各半,除了正常对照组外,其余各组小鼠接受5Gy的X射线一次性的全身均匀照射。检查各小组小鼠外周血中红细胞数、白细胞数,血小板数,测定超氧化物歧化酶的活力,丙二醛水平,小鼠单核巨噬细胞吞噬功能,小鼠血清溶血素生成能力,计算胸腺和脾脏的脏器指数。结果显示沙棘Vp对辐射后的小鼠血象有明显回升(P<0.05),超氧化物歧化酶的活力显著增强(P<0.05),丙二醛水平下降(P<0.05),胸腺和脾脏的脏器指数升高(P<0.05),并且显著提高小鼠血清溶血素生成能力,增强小鼠单核巨噬细胞吞噬功能。本研究说明,沙棘Vp对X射线辐射损伤小鼠具有一定的增强免疫及保护作用。

This study was aimed to explore the protective and immunoregulation effects of sea buckthorn Vp on mice injured by X-ray radiation. 150 mice were randomly divided into 6 groups as follows：normal control group （NC） , model control group （MC） ,positive control group （PC）, Vp low-dosage group （7 mg/kg,was equal of lg herbs）, Vp medium dosage group （ 14 mg/kg）, Vp high-dosage group （28 mg/kg）. In addition to the positive control group, the other groups of mice received a one-time X-ray body of uniform illumination 5Gy. The number of red blood cells, white blood cell count,platelet count in peripheral blood of mice were then determined. The SOD and MDA activity were also deter- mined. The indexes of thymus and spleen were calculated. It was showed that sea buckthorn Vp can significantly increase quantity of hemogram （ P 〈 0.05 ）, activity of liver SOD （ P 〈 0.05 ）, thymus index and spleen index （ P 〈 0.05 ）, and decrease levels of MDA in liver（ P 〈 0.05 ）. In conclusion, sea buckthorn Vp had significant ant-oxidant and immunoreg- ulation effect on mice injured by X-ray radiation.