摘要
[目的]研究食管鳞状细胞癌组织中miR-34a表达水平及其临床意义。[方法]采用实时反转录聚合酶链反应(reverse transcription-polymerase chain reaction,RT-PCR)技术检测75例食管鳞状细胞癌组织及配对癌旁组织中miR-34a的表达水平,2-ΔΔCt法计算食管鳞状细胞癌组织中miR-34a相对表达量,进一步结合临床、病理及随访资料进行统计分析。[结果]miR-34a在75例食管鳞癌组织中相对表达量高于癌旁组织;多因素分析显示,吸烟史和临床分期是食管鳞癌组织中miR-34a表达的独立影响因素。miR-34a低表达组的无进展生存期和总生存期均明显劣于高表达组;多因素分析显示miR-34a低表达是无进展生存期和总生存期缩短的独立影响因素。[结论]食管鳞癌组织中miR-34a表达水平可作为预测预后的一项重要指标。
[Purpose] To investigate the expression of miR-34 a in esophageal squamous cell carcinoma(ESCC) and its clinical significance. [Methods] The expression of miR-34 a in 75 cases with ESCC and adjacent cancer tissues was tested by reverse transcription-polymerase chain reaction(RT-PCR). 2-ΔΔCtmethod was used to calculate the relative quantitative expression of miR-34 a,which was analyzed together with clinicopathological and follow-up data. [Results] The expression of miR-34 a was higher in the tumor tissues than that in cancer adjacent tissues. Multivariate analysis showed that smoking and clinical stage were independent factors influencing the expression of miR-34 a in ESCC.Progression-free survival and overall survival in lower miR-34 a expression group were worse than those in higher miR-34 a expression group. Multivariate analysis indicated that lower miR-34 a expression was independently associated with poorer progression-free survival and overall survival. [Conclusions] Mi R-34 a might be an important indicator for prediction of prognosis of ESCC.
出处
《肿瘤学杂志》
CAS
2016年第12期1026-1031,共6页
Journal of Chinese Oncology
基金
国家自然科学基金资助项目(81172081,81572822)
浙江省科技重大专项(2011C13039-1)
国家卫生计生委科学研究基金-浙江省医药卫生重大科技计划项目(WKJ-ZJ-1505)
浙江省胸部肿瘤(肺、食管)诊治技术研究重点实验室专项基金项目
浙江省卫生高层次创新人才培养工程项目
浙江省新世纪151人才工程重点资助项目