杜衡离体繁殖的研究

Study on shoot tip culture of Asarum forbesii

以马兜铃科植物杜衡为研究材料 ,试图通过茎尖培养来获得愈伤组织 ,继而诱导愈伤组织分化形成杜衡小植株。试验证明 :杜衡茎尖在MS基本培养基附加 6_BA 0 .6mg/l和NAA 0 1mg/l这一激素组合上可诱导茎尖基部形成愈伤组织 ;将愈伤组织分割转接到附加 6_BA 0 2mg/l和NAA 0 0 1mg/l的MS基本培养基上可使愈伤组织分化形成小芽 ;分化形成的芽置于 1/8～ 1/4MS(大量元素减少 ,其余成分不变 )附加 6_BA 0 1mg/l和GA 1mg/l的培养基上 ,可促使芽的正常生长 ;新生芽转接在 1/4MS附加IBA 0 5mg/l培养基上促使其生根。

This paper dealed with the method of shoot tip culture of Asarum forbesii.the results are as follows:the shoot tip of Asarum forbesii could be induced to be callus and furthermore callus could be induced to be buds.Media inducing callus:MS+6_BA0 6mg/l+NAA0 1mg/l;Shooting media:MS+6_BA0 2mg/l+NAA0 01mg/l;Media strengthening buds:1/8～1/4MS+6_BA0 1mg/l+GA1mg/l;Rooting media:1/4MS+IBA0.5mg/l.