上海市浦东新区生鲜食品中食源性致病菌监测

Surveillance of foodborne pathogenic bacteria in raw food in Pudong New Area,Shanghai

目的了解上海市浦东新区生鲜食品中食源性致病菌污染状况。方法 2015年对9类生鲜食品进行致病菌检测,对分离株进行血清分群（型）。结果 1 202份食品中有494份检出致病菌,检出率为41.10%。分离出致病菌587株,副溶血性弧菌（194株）、气单胞菌（134株）、单核细胞增生李斯特菌（131株）为主要致病菌。第二季度检出率最高（47.85%）。虾类检出率最高（64.71%）,不同食品中主要致病菌检出情况存在差异。副溶血性弧菌以O∶1群-O∶5群为主（81.96%）,小肠结肠炎耶尔森菌以O∶8群和O∶5群为主（50.00%）,气单胞菌以维隆气单胞菌为优势菌型（67.91%）,弯曲菌以空肠弯曲菌和结肠弯曲菌为优势菌型（89.29%）,沙门菌血清型分布呈多样性。结论上海市浦东新区生鲜食品中食源性致病菌检出率较高,分布呈多样性和典型的季节性特征,存在多种致病菌混合污染。

Objective To investigate the contamination of foodborne pathogenic bacteria in raw food in Pudong New Area,Shanghai. Methods In 2015,nine kinds of raw food were collected for the detection of pathogenic bacteria,and the isolates were serotyped. Results A total of 41. 10%（ 494 /1 202） food was contaminated,and 587 pathogenic bacteria were isolated. Vibrio parahaemolyticus（ 194）,Aeromonas（ 134） and Listeria monocytogenes（ 131） were the most prevalent pathogens. The highest detection rate was in the second quarter（ 47. 85%） and in shrimp（ 64. 71%）. The distribution of major pathogenic bacteria in foods was different. The dominant serogroup / serotype / species were O ∶ 1-O ∶ 5（ Vibrio parahaemolyticus,81. 96%）,O∶ 8 and O ∶ 5（ Yersinia enterocolitica,50. 00%）,A. veronii（ Aeromonas,67. 91%）,C. jejuni and C. coli（ Campylobacter,89. 29%）. The distribution of Salmonella serotypes was various.Conclusion The contamination of foodborne pathogenic bacteria in raw food was serious in Pudong New Area,Shanghai.The distribution of pathogens was diverse and seasonal,and co-contamination occurred.