2012—2014年无锡市不同来源副溶血性弧菌的病原学特征分析

Etiological analysis of Vibiro parahaemolyticus from different sources in Wuxi during 2012-2014

目的了解近年来无锡市不同来源的副溶血性弧菌的毒力基因携带情况、血清型和耐药性。方法对于分别来源于食源性疾病事件、哨点医院监测标本、外环境监测样品的92株副溶血性弧菌分离株,采用PCR进行毒力基因鉴定,玻片凝集试验进行血清分群,纸片法进行耐药试验。结果 92株副溶血性弧菌中,食源性疾病事件、哨点医院、外环境监测这三种来源的分离株中携带tdh基因的菌株分别占85.7%(12/14)、88.6%(39/44)和14.7%(5/34);食源性疾病事件分离株和哨点医院分离株血清群以O3为主,分别占比85.7%(12/14)和65.9%(29/44),外环境监测分离株无优势血清群;食源性疾病事件、哨点医院、外环境监测这三种来源的分离株中耐氨苄西林的菌株分别占35.7%(5/14)、65.9%(29/44)和11.8%(4/34)。不同来源的菌株对氯霉素、氨苄西林/舒巴坦、庆大霉素、亚胺硫霉素都表现为100.0%敏感。结论 2012—2014年无锡市从食源性疾病事件、哨点医院监测标本和外环境监测样品分离出的副溶血性弧菌在毒力基因、血清群、耐药情况上均有较大差异。建议结合耐药试验结果,在无锡市临床治疗副溶血性弧菌感染病例时,抗生素使用首选氯霉素、氨苄西林/舒巴坦、庆大霉素、亚胺硫霉素。

Objective To investigate the virulence gene,serotype and antibiotic resistance of Vibiro parahaemolyticus from different sources in Wuxi. Methods For 92 strains of Vibiro parahaemolyticus,PCR was used to detect virulence gene. Serotype was determined by serum agglutination test. Antibiotic resistance was performed by disc diffusion method.Results The detection rate of tdh gene among food poisoning strains was 85. 7%（ 12 /14）. The detection rate of tdh among sentinel hospital strains was 88. 6%（ 39 /44）. But the detection rate of tdh among environment strains was only14. 7%（ 5 /34）. Serotype O3 was found to be the dominant serotype among the strains from food poisoning（ 85. 7%,12 /14） and sentinel hospital（ 65. 9%,29 /44）,while there was no dominant serotype found in environment strains. The rate of ampicillin resistance among food poisoning strains was 35. 7%（ 5 /14）. The rate of ampicillin resistance among sentinel hospital strains was 65. 9%（ 29 /44） and was 11. 8%（ 4 /34） among environment strains. All strains were100. 0% sensitive to ampicillin / sulbactam,gentamycin,iminodiaeetic,thienamycin. Conclusion The virulence gene,serotype and antibiotic resistance of V. parahaemolyticus isolated from different sources were different. Ideal antibiotic for treatment of V. parahaemolyticus were ampicillin / sulbactam,gentamycin,iminodiaeetic and thienamycin.