绝经后骨质疏松症肾阴虚证的免疫蛋白相关研究

Analysis of immunoprotein expression in postmenopausal osteoporosis patients with Kidney Yin deficiency

目的探讨绝经后骨质疏松症肾阴虚证与LRG1、SRC蛋白表达变化的关联性。方法随机选择绝经后骨质疏松症患者,中医辨证肾阴虚证组25例,30例健康绝经后妇女为对照组,Western-blot法检测外周血LRG1、SRC的蛋白表达。结果肾阴虚证组LRG1蛋白表达水平(1.2391±1.5860)高于对照组(0.8033±0.7485),有升高趋势,差异无统计学意义(Z=-0.085,P=0.933),肾阴虚证组SRC蛋白表达水平(0.7873±0.6275)与对照组(0.3712±0.4064)比较,差异有统计学意义(Z=-2.874,P=0.004)。结论骨质疏松症肾阴虚证与SRC蛋白表达升高相关联。

Objective To investigate the protein expression of LRG1 and SRC in postmenopausal osteoporosis patients with Kidney Yin deficiency. Methods Postmenopausal osteoporosis patients were randomly selected. Thirty women with normal bone mineral density acted as controls,and 25 women with Kidney Yin deficiency syndrome group were the study group. The protein expression of LRG1 and SRC in peripheral blood was detected by Western-blot method. Results The expression level of LRG1 protein in Kidney Yin deficiency group（1. 2391 ＋ 1. 5860） was higher than that of the control group（0. 8033 ＋ 0. 7485）,but the trend of increase did not reach statistical significance level（Z =- 0. 085,P = 0. 933）. The expression level of SRC protein was significantly higher in Kidney Yin deficiency patients（0. 7873 ＋ 0. 6275） than the controls（0. 3712 ＋ 0. 4064）（Z =- 2. 874,P =0. 004）. Conclusion Osteoporosis patients with Kidney Yin deficiency have increased expression of SRC protein.