股密葆方对大鼠激素性股骨头坏死脂质代谢影响的实验研究

Lipid metabolism research on Gumibao Recipe in treating Steroid-induced Necrosis of the Femoral Head in rats

目的探讨股密葆方对大鼠激素性股骨头坏死的脂质代谢的影响,以揭示其作用的部分机制。方法清洁级SD大鼠50只,分为空白组(A组)、模型组(B组)、股密葆方高、中、低剂量组(C、D、E组),每组10只,雌雄各半。应用大肠杆菌内毒素联合激素注射的造模方法构建动物模型。每组按实验设计行药物干预。于第4周末进行相关数据的观察和比较。结果与模型组相比,股密葆方可以降低总胆固醇(TG)、血清甘油三脂(TC)、低密度脂蛋白(LDL)的水平,提高高密度脂蛋白(HDL)水平,与模组比较,差异具有统计学意义(P<0.05)。透射电镜结果显示,模型组骨细胞结构破坏,胞浆内充满了脂肪滴,无完整的细胞结构,胞核碎裂消失,骨细胞变性坏死;中药组的骨细胞内细胞器结构较模型组完整,髓腔内脂肪栓子较模型组少见。结论脂质代谢紊乱与激素性股骨头坏死关系密切,股密葆方可以调节激素性股骨头坏死大鼠模型的脂质水平,降低血脂,改善高脂血症,一定程度上延缓股骨头坏死的进展。

Objective To investigate the effects of Gumibao Recipe on lipid metabolism in Steroid-induced Necrosis of the Femoral Head（SINFH） in rats,in order to explore its partial mechanisms for treating Steroid-induced Necrosis of The Femoral Head. Methods Fifty SD rats were randomly assigned to five groups： blank control group,model group,Gumibao Recipe groups（High,medium and low-dose groups,10 rats in each group,with half male and half female）. SINFH models were established according to S. Okazaki＇s technique. All the interventions lasted for 4 weeks by gavage. The data on lipid metabolism were determined. Results Compared with the model group,Gumibao Recipe could reduce the serum levels of TC,TG,LDL and enhanced the level of HDL（P〈0. 05）. TEMultrastructure observation showed that compared with the model group,the bone cell morphological of Gumibao recipe was better. Conclusion Lipid metabolism disorder is closely related to SINFH,and Gumibao recipe could regulate lipid metabolism,improve dyslipidemia,and delay the development of SINFH.