雷奈酸锶对大鼠快速扩弓过程中腭中缝BMP-2表达的影响

Effects of strontium ranelate on the expression of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion

目的:研究雷奈酸锶对大鼠快速扩弓过程中腭中缝骨形态发生蛋白2(bone morphogenetic protein-2,BMP-2)表达的影响。方法:选取36只6周龄雄性Wistar大鼠,随机分为A、B、C 3组,每组12只,其中,A组为对照组。将机械扩弓装置置于B、C 2组大鼠上颌两侧第一、二磨牙之间,并加力100 g,A组不做处理。C组大鼠每天灌服600 mg/kg雷奈酸锶溶液,B组灌服相同剂量的生理盐水。分别于加力后4、7、10 d分批处死大鼠,用免疫组织化学染色法检测大鼠腭中缝组织中BMP-2的表达,并利用Image-pro plus粘结对切片吸光度进行分析,采用SPSS19.0软件包对数据进行统计学分析。结果:免疫组织化学染色显示,B组大鼠腭中缝组织中BMP-2的表达量在第4天时显著高于A组(P<0.05),而在第7天和第10天时与A组无显著差异(P>0.05);C组大鼠腭中缝组织中BMP-2的表达量在各个时间点均显著高于其余2组(P<0.05)。结论:雷奈酸锶能够促进大鼠快速扩弓过程中BMP-2的表达量和表达时长,可能促进了快速扩弓过程中腭中缝的新骨形成。

PURPOSE： To investigate the effects of strontium ranelate on the expression of BMP-2 during rapid maxillary expansion. METHODS： Thirty-six male 6-week- old male Wistar rats were selected. They were randomly divided into 3 groups. Group A was designed as a control group. An expanded application producing 100 g force was fixed between the first and the second molar on both sides of the rats in group B and group C. 600 mg/kg strontium ranelate was given to the rats in group C daily via an orogastric route, while the equal normal saline was given to the rats in group B. Then the rats were sacrificed on day 4, 7 and 10. The expression of BMP-2 which is a sign of bone formation was detected with immunohistochemical staining and analyzed with Image-pro plus 5.0. SPSS19.0 software package was used for statistical analysis. RESULTS： The expression of BMP-2 in the midpalatal suture was significantly greater in group B than that in group A on day 4 （P〈0.05）; however, there was no significant difference between group A and group B on day 7 and 10 （P〉0.05）. The expression of BMP-2 in the midpalatal suture in group C was significantly greater than that in the other two groups at each time point （P〈0.05）. CONCLUSIONS： Strontium ranelate promotes the expression time and quantity Of BMP-2 in the mid-palatal suture of rats during rapid maxillary expansion and may accelerate bone formation during rapid maxillary exoansion.