LIF/Apelin mRNA在妊娠高血压患者胎盘组织中的表达与围产儿结局的关系探究

Correlation between Expression of LIF/Apelin mRNA in placentatissue of hypertensive patients during pregnancy and perinatal outcome

目的 探讨白血病抑制因子（leukaemia inhibitory factor,LIF）和Apelin mRNA在妊娠高血压患者胎盘组织中的表达与围产儿结局的关系。方法 收集2012-2014年来我院就诊的妊娠期高血压患者160例,同期收集来我院产前检查的80例健康孕妇,分别经实时荧光定量PCR（qPCR）检测其胎盘组织LIF和Apelin mRNA的表达差异,分析其表达水平差异与患者围产儿结局的相关性。结果 妊娠高血压患病组胎盘组织中LIF和Apelin mRNA表达水平都低于健康组,差异均有统计学意义（P〈0.05）。采用Logistic回归分析妊娠高血压与LIF和Apelin mRNA表达水平的相关性,相关性检验有统计学意义（P〈0.05）,最大似然估计值和OR值均〈0。进一步针对围产儿结局进行相关性分析,亦有统计学意义（P〈0.05）,LIF和Apelin mRNA平均表达评分与平均新生儿体质量及平均新生儿评分相关系数介于0.721~0.836之间。结论LIF和Apelin mRNA在妊娠高血压患者胎盘组织中的表达及与围产儿不良结局呈负相关,可用于评估孕妇病情及围产儿结局。

Objective To investigate the correlations between the expression of leukaemia inhibitory factor （LIF） and Ape- lin mRNA in placenta tissue of patients with hypertension in pregnancy and the perinatal outcome. Methods 160 cases of patients with hypertension in pregnancy trated at our hospital from January 2012 to December 2014 were collected while another 80 cases of healthy pregnant women were collected during the same period. The expressions of LIF and Apelin mRNA were detected by real- time fluorescence quantitative PCR （qPCR）. Logistic regression analysis was used to analyze the correlation between the levels of LIF/Apelin mRNA and hypertension in pregnancy. Results The expression of LIF and Apelin mRNA in placenta tissue of pregnant women with gestational hypertension group was lower than that of healthy pregnant women group（ P〈0.05 ）. The correlation between the expression level of LIF/Apelin mRNA and the perinatal outcome was statistically significant （ P〈O. 05 ）. The maximum likeli- hood estimates and OR values were less than 0. The correlation coefficient of LIF/Apelin mRNA average expression score and mean birth weight and average neonatal score ranged from O. 721 toO. 836. Conclusion The expression of LIF/Apelin mRNA in placenta tissue of patients with hypertension in pregnancy is negatively correlated with the adverse perinatal outcome.