血脑屏障中MMP-2、TIMP-2水平变化与脑神经胶质瘤侵袭能力的关系

Relationship of Blood MMP-2、TIMP-2 Brain Barrier and Glioma Invasion Ability

目的探讨血脑屏障紧密连接变化对脑神经胶质瘤侵袭能力的影响,为评价病情进展提供依据。方法选择到我院神经外科就诊的87例胶质细胞瘤患者,WHOⅠ级29例,Ⅱ级26例,Ⅲ级21例,Ⅳ级11例,对各级患者血清S-100、胶质纤维酸性蛋白(GFAP)、基质金属蛋白酶-2(MMP-2)及其抑制剂(TIMP-2)进行检测。结果Ⅱ级患者S-100及GFAP水平显著高于Ⅰ级患者(P<0.05),Ⅲ级患者S-100及GFAP水平显著高于Ⅰ、Ⅱ级患者(P<0.05),Ⅳ级患者S-100及GFAP水平显著高于Ⅰ、Ⅱ、Ⅲ、Ⅳ级患者(P<0.05)。Ⅰ、Ⅱ、Ⅲ、Ⅳ级患者MMP-2/TIMP-2分别为0.107±0.016、0.147±0.018、0.211±0.030、0.409±0.051,各级之间比较,差异均有显著性(P<0.05)。S-100、GFAP与MMP-2、MMP-2/TIMP-2均呈显著正相关(P<0.05)。结论随神经胶质瘤分级进展,血脑屏障紧密连接被破坏且与脑神经胶质瘤侵袭能力密切相关。

Objective To investigate the effects of tight junction of the blood-brain barrier on the glioma invasion ability. Methods 87 cases of neurosurgery glioblastoma patients including grade Ⅰ 29 cases,26 cases of patients with grade Ⅱ , Ⅲ grade 21 cases , Ⅳ grade 11 patients were enrolled. Serum levels in patients of S-100, GFAP, MMP-2, TIMP-2 were detected. Results S- 100 ,GFAP in patients with grade Ⅱ compared with I appeared significantly higher level （P〈0.05）, S-100, GFAP of 11 grade pa- tients compared with gradeⅠ,Ⅱ grade were significantly higher（ P〈0.05 ）, S-100, GFAP of Ⅳ grade compared with gradeI, llgrade , grade m were significantly（P〈0.05）. MMP-2 / TIMP-2 in Ⅱ level patients compared with class Ⅰ was significantly higher（P〈0. 05 ）, MMP-2 and TIMP-2 in Ⅲ grade patients compared with grade Ⅰ were any significant different（P〈0.05 ）, MMP-2 / TIMP -2 compared with grade Ⅰ ,grade Ⅱ patients were significantly higher（P〈0.05） ,MMP-2 and TIMP-2 and MMP-2 / TIMP-2 of Ⅳ grade compared with grade Ⅰ,Ⅱ,Ⅲ were any significant different（P〈0.05 ）. S-100, GFAP and MMP-2, MMP-2 / TIMP-2 were significantly correlated （P〈0.05）, while showing no effects on TIMP-2 （P〉0.05）. Conclusion With the progress of glioma grading ,the tight junction of BBB were destroyed and were closely related to glioma invasion ability.