银杏叶提取物对急性期溃疡性结肠炎模型小鼠防御素的调节作用

Regulative Effects of Gingko Biloba Extract for Acute Mouse Model Alexln of Acute Ulcerative Colitis

目的：银杏叶提取物（gingko biloba extract,EGB 761）对急性期溃疡性结肠炎模型小鼠防御素的调节作用。方法：30只小鼠随机分为正常对照组、模型组、EGB761组,建立急性溃疡性结肠炎动物模型;观察小鼠一般情况及结肠病理学变化,应用免疫组织化学染色、Western blot技术检测结肠组织的防御素~1（HBD-1）和防御素~2（HBD-2）的蛋白表达情况。结果：经EGB 761治疗后模型小鼠的一般情况及病理组织学均有明显改变,与模型组比较,EGB 761组结肠组织HBD-1mRNA和蛋白表达明显增加,但HBD-2mRNA和蛋白表达明显下降。结论：可见EGB761对急性期溃疡性结肠炎模型小鼠结肠组织中的防御素具有调节作用。

Objective： To investigate the the effects of Gingko Biloba Extract on the human defensins in the acute experimental colitis. Methods： Thirty mice were randomly grouped as follows： controlled group,model group,EGB 761 group,acute colitis were established,general condition and colon pathological changes were observed,the expressions of HBD- 1and HBD-2mRNAand proteins were detected by real-time Q-PCR,western blot. Results： General condition and colon pathological were changed in the EGB group compared with that of the model group; the proteins and mRNA of expressions of HBD- 1and HBD-2were significantly increased in the EGB group compared with that of the model group by western blot,real-time Q-PCR. Conclusions： The administration of EGB may be adjusted the human defensins in the acute experimental colitis.