EMS诱变后甘蓝型油菜植株性状、种子粒重与含油量变异分析

Phenotypic variation of plant,seed-weight and oil content in Brassica napus induced by EMS

为创建突变体库,丰富甘蓝型油菜的遗传资源,利用两种浓度的甲基磺酸乙酯(EM_S)溶液处理甘蓝型油菜双低品种浙油18的种子。经田间观察,M_2植株在苗期、花期有叶色、叶形、茎色、花瓣颜色、花瓣形状、花瓣数目、雄性不育和开花期明显变异株。测定M_3种子千粒重与含油量,获得28份大粒(最大千粒重为7.32g)、9份高油(最高含油量为52.80%)材料。0.3%EMS处理后,M_2植株中叶片、花器官、株型与茎秆性状的突变频率分别为1.282%、8.546%和0.977%;M_3种子千粒重和含油量均值均小于对照,但不显著。0.6%EMS处理后,M_2植株相应性状的突变频率更高,M_3种子千粒重和含油量均值都极显著小于对照(P<0.01)。

For constructing mutant library and enriching genetic resource of Brassica napus, ethyl methane sulfonate （EMS） was applied on seeds of double low B. napus cultivar Zheyou 18. Mutants with abnormal appearance of leaf color, leaf shape, stem color, petal color, petal shape, petal number, male sterility, and booming date were observed in M2 population. 28 large-seed mutants and 9 high-oil mutants were determined for thousand-seed weight （TSW） and oil content in M3 seeds. The greatest TSW was 7.32g, and the highest oil content was 52.80%. Phenotypic variation of M2 plants showed that the mutation frequencies under 0.6% EMS was higher than those under 0.3% EMS. Average M3 TSW and oil content were highly significantly lower （P〈0.01） than those of wild type under 0.6% EMS treatment.