摘要
利用分子生物学技术扩增藏羊腐蹄病病料中坏死杆菌和节瘤拟杆菌的特异性基因片段。结果显示:坏死杆菌lkt A基因和节瘤拟杆菌fim A基因序列与Genebank中JX678850.1和X52407的序列同源性分别为99%和95%,表明青海省藏羊腐蹄病病原中有坏死杆菌和节瘤拟杆菌存在,为今后防治提供参考依据。
This study amplied the specific gene fragments of Fusobacterium necrophorum and Dichelobacter nodosus from swab samples ofTibetan sheep by molecular biology method. Results showed the homology of C4F - FF1 - Cll and JX678850.1 is 99% ; the homology ofC33D - DF1 - B07 and X52407 is 95% by the NCBI blast analysis. This study results showed that the pathogens of footrot disease are Fu-sobacterium necrophorum and Dichelobacter nodosus. The study aims to provide reference for the prevention and control work.
出处
《青海畜牧兽医杂志》
2016年第6期1-4,共4页
Chinese Qinghai Journal of Animal and Veterinary Sciences
基金
基金项目:国家绒毛用羊产业技术体系(CARS-40-4B)
关键词
腐蹄病
坏死杆菌
节瘤拟杆菌
分子生物学技术
Footrot disease
Fusobacterium necrophorum
Dichelobacter nodosus
Molecular biology method
