绿色木霉内切几丁质酶基因的克隆及其毛壳菌转化

CLONING OF AN ENDOCHITINASE GENE FROM TRICHODERMA VIRIDE AND ITS TRANSFORMATION INTO CHAETOMIUM GLOBOSUM

利用PCR方法从Trichoderma viride的基因组DNA中克隆了一个42kDa的内切几丁质酶基因,扩增的长度为1672bp,其中包含了启动子和mRNA的编码区。将该基因与来自构巢曲霉的色氨酸启动子相连后,通过原生质体转化将该基因导入球壳毛壳菌CG10。内切几丁质酶活性的测定结果表明,约1/3转化子的内切几丁质酶活性得到了明显提高。本实验为利用基因工程方法提高毛壳菌的生防能力打下了较好的基础。

An endochitinase encoding gene was cloned from the genome DNA of Trichoderma viride. The length of this DNA fragment is 1672 bp and contains a promoter and coding region for mRNA. After ligating it with the promoter and terminator of Trp synthetase from Aspergillus nidulan, this gene was introduced into Chaetomium globosum CG10. It was indicated about one third transformants increased their endochitinase activity significantly. This experiment lays some foundation and present a potential way for improvement of biocontrol activity of Chaetomium globosum with genetic engineering method.