摘要
由褐腐真菌的典型菌株——密粘褶菌Gloeophyllum trabeum的胞外培养液中分离纯化得到一能还原Fe3+,产生羟基自由基HO˙的多肽组分(称作Gt因子)。 采用HO˙特异性的抑制剂硫脲,对Gt因子产生的HO˙在纤维素降解中的作用进行了对照研究,结果表明Gt因子及其产生的HO˙在纤维素降解中起着重要的作用,为褐腐菌HO˙氧化降解纤维素机制假说的确立提供了一些依据。
A peptide(named Gt factor) was isolated from the extracellular culture of Gloeophyllum trabeum, the typical brown-rot fungus. Gt factor could reduce Fe3+ to Fe2+ and mediated the production of HO˙ Effects of HO˙on cellulose degradation was comparatively studied with thiourea used as HO˙- specific inhibitor. All results showed that HO˙ produced by Gt factor did have some effects on cellulose degradation, it provided some evidence of HO˙be involved in oxidative mechanism of cellulose degradation by brown-rot fungi.
出处
《菌物系统》
CSCD
北大核心
2002年第3期400-405,共6页
Mycosystema
基金
国家自然科学基金资助项目(39970004)
关键词
密粘褶菌
纤维素
HO^·氧化机制
硫脲
Gloeophyllum trabeum, cellulose, HO˙-involved oxidative mechanism, thiourea
