4种microRNAs在寻常型银屑病患者外周血中的表达

The Expression of Four microRNAs in Peripheral Blood of Patients with Psoriasis Vulgaris

目的:研究寻常型银屑病患者外周血中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p的表达情况。方法:采用实时荧光定量PCR技术检测60例寻常型银屑病患者和6例正常人外周血中的4种miRNAs表达,并对患者进行PASI评分,采用spearman检验分析4种miRNAs表达量与银屑病PASI评分的相关性。结果:与正常人比较,寻常型银屑病患者外周血中miR-30e-5p、miR-192-5p、miR-17-3p、miR-1227-5p表达下调,下调程度与PASI评分呈负相关(r值均<0,P值均<0.05)。结论:4种miRNAs可能可作为银屑病患者潜在外周血生物标志物,其表达量可用于评价银屑病病情严重程度。

Objective：To study the expression and clinical significance of miRNA in peripheral blood of psoriasis vulgaris. Methods： Four representative miRNAs （ miR-30e-5p, miR-192-5p, miR-17-3p,miR-1227-5p） were selected in this study. Their expression levels in serum of 60 patients with psoriasis vulgaris and 6 healthy people were monitored by real-time PCR, and PASI score in patients were evaluated. Then the relationship of the expression of four miRNAs and the PASI score was analyzed by Spearman Test. Results： Compared with healthy people, in the peripheral blood of patients with psoriasis vulgaris, expression levels of miR-3Oe-Sp, miR-192-5p, miR-17-3p and miR-1227-5p were all down-regulated, and were negatively correlated with the PASI score （ all P 〈 0. 05 ）. Conclusions ： The expression levels of serum miR-30e-5p, miR-192-5p, miR-17-3p,miR-1227-5p can be used as biomarkers for Psoriasis Vulgaris.