慢性荨麻疹患者外周血中CD8^+CD28^-T细胞检测结果分析

Analysis of CD8^+CD28^-T Cell in Peripheral Blood from Patients with Chronic Urticaria

目的:探讨CD8^+CD28^-T细胞在慢性荨麻疹患者致病机制中的作用。方法:收集临床慢性荨麻疹(CU)病例83例,同时设立对照组64例进行比较。应用ELISA法检测研究对象血清中抗Ig E抗体、抗FcεRⅠ抗体浓度,流式细胞仪检测外周血中CD3^+、CD4^+、CD8^+、CD8^+CD28^-、CD4^+CD25^+T细胞比例,分析检测结果。结果:83名CU中,23例抗Ig E抗体为阳性,占27.7%(27/83);31例抗FcεRⅠ抗体为阳性,占37.3%(31/83)。CU患者外周血CD8^+T细胞比例、CD8^+CD28^-T细胞比例低于正常对照组(P<0.05),CD4^+/CD8^+比值、CD4^+CD25^+T细胞比例均高于对照组(P<0.05)。结论:CU患者机体外周血CD8^+CD28^-T细胞、CD4^+CD25^+T细胞比例与对照组相比存在差异,CD8^+CD28^-T细胞比例降低也可能是CU致病机制之一。

Objective ：To explore the possible pathogenic mechanisms of CD8^＋ CD28^-T cells in patients with chronic urticaria （CU）. Methods：The levels of serum anti-IgE antibody and anti- Fc^R I antibody were measured by ELISA and the population of CD3^＋ , CD4^＋ , CD8^＋ , CD8^＋ CD28^- and CD4^＋ CD25 ^＋ T cells in peripheral blood was detected by flow cytometry in 83 patients with CU and 64 healthy controls. Results：Of the 83 patients with CU, 23 （27.7%） was positive for anti-IgE antibody and 31 （37.3%） was positive for anti-FcεR I antibody. The percentage of CD8^＋ and CD8^＋ CD28^-T cells in peripheral blood of patients with CU was lower than those in healthy controls （P 〈 0. 05 ）. The proportion of CD4^＋ / CD8^＋ and CD4^＋CD25^＋ T cells in patients with CU was higher as compared to those in healthy controls （P 〈 0.05 ）. Conclusion：The abnormal proportion of CD8^＋CD28^- and CD4^＋ CD25^＋ T cells in peripheral blood of patients with CU may be involved in the pathogenesis underlying CU.