肾虚及正常大鼠的正畸致牙根吸收易感性比较及组织内白细胞介素17的表达差异

Root resorption and interleukin-17 expression in a rat model of kidney deficiency uring orthodontic treatment

背景:正畸致牙根外吸收的发生机制目前尚不明确,且它有较大的个体差异。肾虚体质已被证明与多种细胞因子介导的骨性疾病有直接联系,白细胞介素17为目前研究出的参与牙根外吸收的重要细胞因子之一。明确肾虚体质是否与牙根吸收相关及白细胞介素17是否参与其中,有助于正畸致牙根吸收的病因学研究。目的:探讨正畸移动大鼠牙过程中肾虚体质及白细胞介素17与牙根吸收的关系。方法:选择36只Wistar大鼠,随机分为2组,每组18只。肾虚组应用大鼠药物诱导法建立肾虚模型,对照组给予生理盐水。肾虚模型建立后,所有大鼠右侧上颌建立正畸加力模型,左侧上颌不加力。正畸加力3,7,14 d时全麻下分批处死大鼠,苏木精-伊红染色、免疫组化检测观察第一磨牙近中根压力侧组织形态及白细胞介素17的表达变化。结果与结论:1组织学结果:肾虚加力组牙根压力侧吸收,牙釉质表面不同程度的破骨细胞吸收陷窝,吸收程度随加力时间延长而加重;对照加力组牙槽骨吸收、牙周膜增宽;肾虚不加力组及对照不加力组未见牙槽骨及牙根吸收;2免疫组化结果:白细胞介素17在肾虚组加力组牙根压力侧表达高于对照加力组,在肾虚不加力组的表达高于对照不加力组;3结果表明,肾虚体质下进行正畸治疗易发生牙根吸收,且肾虚体质较正常体质更易产生白细胞介素17,其造成牙根吸收的机制可能与白细胞介素17相关。

BACKGROUND：The mechanism underlying orthodontic-induced external root resorption is not yet clear, and it differs individual y. Kidney deficiency has been proved to be related to bone diseases which mediated by different cytokines. Interleukin-17 is an important cytokine involved in external root resorption. So figuring out whether kidney deficiency and interleukin-17 are related to root resorption wil be helpful for etiological research. OBJECTIVE：To explore the relationship between kidney deficiency physique, interleukin-17 and root resorption during orthodontic treatment in rats. METHODS：Thirty-six Wistar rats were selected and equivalently randomized into two groups, fol owed by modeled into kidney deficiency （kidney deficiency group） or injected with normal saline （control group）, respectively. Afterwards, the right maxil ary of each rat served as an orthodontic force model, and the left maxil ary as a non-orthodontic force model. Al rats were respectively sacrificed under general anesthesia at the 3, 7 and 14 days after given orthodontic force. Then, the mesial surface of the root of maxil ary first molars and the expression level of interleukin-17 were observed through hematoxylin-eosin staining and immunohistochemical method. RESULTS AND CONCLUSION：Histological observation showed that significantly increasing root resorption in a time-dependent manner could be observed, and there were various absorbed lacunae of osteoclasts on the enamel in the kidney deficiency orthodontic force group. The alveolar bone resorption and widened periodontal membrane appeared in the control orthodontic force group. While no remarkable root and alveolar bone resorptions were found in the other two non-orthodontic force groups. The expression level of interleukin-17 in the kidney deficiency orthodontic force group was higher than that in the control orthodontic force group;the expression level of interleukin-17 in the kidney deficiency non-orthodontic force group was higher than that in the control non-orthodontic force group. In conclusion, kidney deficiency patients are easy to develop root resorption, the mechanism of which is maybe relevant to the upregulation of interleukin-17.