多亮氨酸重复区免疫球蛋白样蛋白1对U251胶质瘤细胞基因表达的影响

Effect of Leucine-rich Repeats and Immunoglobulin-like Domains 1 on Genes Expression in Glioma U251 Cells:a DNA Microarray Analysis

目的运用基因芯片技术研究多亮氨酸重复区免疫球蛋白样蛋白1(leucine-rich repeats and immunoglobulin-like domains 1,LRIG1)对U251胶质瘤细胞基因表达的影响。方法提取正常U251细胞和转染了LRIG1的U251细胞的RNA,并反转录为cDNA,加用cy3、cy5染色标记后使用Roche-Nimble Gen人全基因组表达谱芯片检测LRIG1对U251胶质瘤细胞基因表达的影响。结果转染LRIG1的U251细胞相对U251细胞,差异基因为808条,其中上调基因有397条,下调基因有411条,包括细胞骨架、细胞增殖、细胞凋亡、生长代谢等相关基因。结论基因芯片能够高效地初步检测差异基因表达,有助于揭示LRIG1作用于胶质瘤细胞的分子机制。

Objective To study the effect of leucine - rich repeats and immunoglobulin - like domains 1 （ LRIG1 ） on genes expres- sion in glioma U251 ceils. Methods The total RNAs were isolated from the empty U251 cells and the LRIG1 vector transfected U251 cells. The corresponding cDNAs were obtained by reaction of reversely transcription. The cNDA products were labeled with cy3 and cy5 fluorescence dye before microarray hybridization. Differential expression of genes in U251 cells and LRIG1 -U251 cells were detected by methods of Roche - NimbleGen DNA microarray. Results Compared to the empty U251 ceils, there were 808 differential expressing genes, which consisting of 397 up - regulating genes and 411 down - regulating genes. These genes involve cell processes of cytoskeleton, apoptosis, proliferation, cell growth and metabolism. Conclusion DNA microarray can be an effective method for screening differential genes. The obtained differential genes are definitely helpful for further investigating the molecular mechanism of how LRIG1 acts on glioma cells.