摘要
背景:不同类型心脏病患者机体的差异是否会影响干细胞的特征和表现尚不清楚。目的:探讨不同类型心脏病患者骨髓间充质干细胞基本生物学特性。方法:纳入27例冠心病患者与20例非冠心病患者,收集胸骨骨髓液采用密度梯度离心法进行骨髓间充质干细胞体外培养,观察两组细胞的形态,流式细胞术鉴定CD13、CD34、CD44、CD45、CD54、CD106阳性表达率,MTT法检测细胞生长增殖情况,描绘两组细胞的生长曲线。结果与结论:(1)两组患者骨髓间充质干细胞均呈现出长梭形纤维状,形态无明显差异;(2)两组患者骨髓间充质干细胞CD34、CD455阳性表达率均小于3.0%,CD13、CD44阳性率均大于95.0%。非冠心病组CD54、CD106阳性表达率显著低于冠心病组(P<0.05);(3)两组细胞的体外生长曲线基本一致,非冠心病组的增长速度略快,但与冠心病组比较差异无显著性意义;(4)结果表明,不同类型心脏病患者的骨髓间充质干细胞形态和增殖情况均无明显差异,但是与功能相关的某些蛋白表达存在一定的差异。
BACKGROUND:It is unclear whether the body differences in patients with different types of heart diseases affect the characteristics and performance of stem cel s. OBJECTIVE:To explore the biological characteristics of bone marrow mesenchymal stem cel s from patients with different types of heart diseases. METHODS:Bone marrow mesenchymal stem cel s were extracted using density gradient centrifugation from the bone marrow of 27 patients with coronary heart diseases and 20 patients with other heart diseases. Cel morphology was observed in the two groups. CD13, CD34, CD45, CD54, CD106 and CD44 positive expression was detected by flow cytometry. Cel proliferation was detected by MTT method, and the in vitro cel growth curves of the two groups were described. RESULTS AND CONCLUSION:The bone marrow mesenchymal stem cel s of the two groups showed a long spindle shape, and there was no significant difference in the cel morphology between the two groups. In al the patients, the positive rates of CD34 and CD455 were less than 3.0%, while the positive rates of CD13 and CD44 were higher than 95.0%. However, the positive rates of CD54 and CD106 were higher in patients with coronary heart disease as compared with those with other heart diseases (P〈0.05). The in vitro growth curves of cel s in the two groups were basical y consistent, and the cel proliferation was only a little higher in the patients with other heart diseases compared with those with coronary heart disease. Experimental results show that different types of heart diseases in patients have no influence on morphology and proliferation of bone marrow mesenchymal stem cel s, but some function-related proteins may exhibit certain difference in their expressions.
作者
侯永兰
Hou Yong-lan(Department of Cardiology, Xinxiang Central Hospital, Xinxiang 453000, Henan Province, Chin)
出处
《中国组织工程研究》
CAS
北大核心
2016年第50期7481-7486,共6页
Chinese Journal of Tissue Engineering Research