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玻璃酸钠壳聚糖纳米粒对烧伤角膜新生血管生长的影响 被引量:4

Effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea
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摘要 背景:将玻璃酸钠制作成由壳聚糖纳米粒包裹而成的药物,可以使药物更好地作用于烧伤角膜。目的:验证玻璃酸钠壳聚糖纳米粒对烧伤角膜新生血管生长的影响。方法:将30只SD大鼠随机分为3组,模型组、实验组制备碱烧伤角膜模型,正常对照组不造模,实验组在造模后2周后滴注玻璃酸钠壳聚糖纳米粒悬液治疗,1次/d,10μL/次,正常对照组与模型组给予等量生理盐水,持续4周。4周后,裂隙灯观察眼角膜新生血管动态生长情况;ELISA法测定白细胞介素6和肿瘤坏死因子α含量;苏木精-伊红染色观察角膜病理学变化;聚合酶链式反应实时荧光定量PCR检测检测血管内皮因子和环氧化酶2 mRNA表达量。结果与结论:(1)与正常对照组比较,模型组角膜新生血管面积、白细胞介素6、肿瘤坏死因子α、血管内皮生长因子、环氧化酶2水平显著升高(P<0.05,P<0.01);与模型组比较,实验组角膜新生血管面积、白细胞介素6、肿瘤坏死因子α、血管内皮生长因子、环氧化酶2水平显著降低(P<0.05);(2)模型组眼角组织有大量炎性细胞及新生血管产生,实验组仅有少量炎性细胞存在;(3)结果表明,玻璃酸钠壳聚糖纳米粒可抑制烧伤角膜新生血管的生长。 BACKGROUND:Chitosan nanoparticles-encapsuled sodium hyaluronate is an effective drug for the burned cornea. OBJECTIVE:To verify the effect of sodium hyaluronate/chitosan nanoparticles on the neovascularization in burned cornea. METHODS:Thirty Sprague-Dawley rats were randomly divided into three groups, and the model of burned cornea caused by base was established in the rats of model and experimental groups, fol owed by respectively treated with 10μL sodium hyaluronate/chitosan nanoparticle suspension and normal saline, once daily, for consecutive 4 weeks. Rats only given normal saline were used as controls. Four weeks later, the dynamic growth of newly formed blood vessels in the cornea was observed using silt lamp. The levels of tumor necrosis factor-αand interleukin-6 were detected by ELISA, histological changes of the cornea were observed by hematoxylin-eosin staining, and the mRNA expression levels of vascular endothelial growth factor and cyclooxygenase 2 were detected by real-time PCR. RESULTS AND CONCLUSION:Compared with the control group, the area of the newly formed blood vessel and the levels of tumor necrosis factor-α, vascular endothelial growth factor and cyclooxygenase 2 were significantly increased in the model group (P〈0.05, P〈0.01). In the experimental group, al above indicators were significantly lower than those in the model group (P〈0.05). There were a large number of inflammatory cel s and neovascularization in the model group, but only few inflammatory cel s in the experimental group. These results show that sodium hyaluronate/chitosan nanoparticles can inhibit the neovascularization in the burned cornea.
出处 《中国组织工程研究》 CAS 北大核心 2016年第52期7803-7808,共6页 Chinese Journal of Tissue Engineering Research
基金 河北省医学科学研究重点课题(zl20140018)~~
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