“通体结香技术”所产沉香提取物对胃肠动力及胃溃疡的作用

Effect of agarwood extracts produced by the whole-tree agarwood-inducing technique on gastrointestinal motility and gastric ulcer

目的评价"通体结香技术"所产沉香提取物对胃肠道功能的影响,为其今后临床应用及产品开发提供科学依据。方法采用炭末法小肠推进实验,观察沉香对小肠推进速率的影响;甲基橙法胃排空实验检测沉香对小鼠胃内甲基橙残留量的影响;采用应激水浸的方法建立大鼠胃溃疡实验模型,考察沉香对大鼠胃溃疡发生情况及溃疡指数的影响。结果单次和多次灌胃给予通体沉香醇提物(150 mg/kg)能显著增强小鼠肠推进和胃排空速率,多次给予市售沉香醇提物450 mg/kg也能显著增强肠推进作用,但它们各自的沉香水提物作用不明显。同时,通体沉香醇提物单次给药能显著抑制大鼠胃溃疡的发生,多次给药(300 mg/kg)对胃溃疡抑制率为(73.1±5.6)%。结论利用"通体结香技术"所产沉香的醇提物具有促进肠推进、胃排空和抑制胃溃疡的作用。

Objective To evaluate the gastrointestinal regulative effect of agarwood extracts produced by the whole-tree agarwood-inducing technique（Agar-Wit agarwood）to make scientific basis for the Agar-Wit agarwood exploration and usage in clinic.Methods Intestinal propelling effect assay marked by carbon powder,and gastric empting effect assay marked by methyl orange were used to evaluate the gastrointestinal effects of Agar-Wit agarwood on mice by single or constant repeated intragastric（ig）administration of water extract and ethanol extract,and then the index of intestinal peristaltic rate and methyl orange relative residual rate were calculated. Gastrtic ulcer model was established under water stress to appraise their protective function on rat stomach and to obtain the gastric ulcer index and inhibitory rate. Results The Agar-Wit agarwood ethanol extract significantly improved intestinal peristalsis and gastric empting function by single or constant repeated ig administration at the dose of 150 mg/kg. The ethanol extract of commercial agarwood also had the similar effect at the dose of 450 mg/kg. But the water extract did not have significant effect. The gastric ulcer assay results showed that the ethanol extract significantly inhibited gastric ulcer happening by single ig administration. Additionally,constant repeated ig administration of ethanol extract showed the consistent results and the gastric ulcer inhibitory rate was（73.1±5.6）% at the dose of 300 mg/kg. Conclusion The Agar-Wit agarwood ethanol extracts have significant intestinal peristaltic effect,gastric empting effect and gastric ulcer inhibitory function.