血清miR-6801在非小细胞肺癌辅助诊断中的临床意义

Clinical significance of serum miR-6801 in auxiliary diagnosis of non-small cell lung cancer

目的探讨血清miR-6801在非小细胞肺癌(NSCLC)患者中的表达水平及其在NSCLC辅助诊断中的临床意义。方法采用实时荧光定量聚合酶链反应(PCR)检测40例健康人和78例NSCLC患者血清中miR-6801的表达量,并构建受试者操作特征(ROC)曲线计算血清miR-6801的灵敏度和特异度。结果 NSCLC患者血清中miR-6801的表达水平显著低于健康者,差异有统计学意义(P<0.05);血清miR-6801在Ⅲ期+Ⅳ期中的表达水平显著低于Ⅰ期+Ⅱ期(P<0.05);血清miR-6801对NSCLC检测的灵敏度为51.9%;miR-6801对Ⅰ期、Ⅱ期NSCLC检测的灵敏度为40.6%。结论 NSCLC患者miR-6801的检测对于NSCLC的辅助诊断和早期诊断有一定的临床应用价值。

Objective To investigate the expression level of serum miR-6801 in non-small cell lung cancer（NSCLC）and its clinical significance in the auxiliary diagnosis of NSCLC.Methods The real-time quantitative PCR method was adopted to examine the expression amount of serum miR-6801 in 78NSCLC patients and 40 healthy controls.The sensitivity and specificity of miR-6801 in the diagnosis of NSCLC were evaluated by receiver operating characteristic（ROC）curve.Results The expression level of miR-6801 in the patients with NSCLC was significantly lower than that in healthy controls with statistical difference（P〈0.05）;the expression level of miR-6801 at stagesⅢ and Ⅳ was significantly lower than that at stagesⅠ and Ⅱ（P〈0.05）;the sensitivity of miR-6801 in detecting NSCLC was 51.9%,and the sensitivities of miR-6801 in the stage I and II of NSCLC was 40.6%.Conclusion The serum miR-6801 detection has a certain clinical application value for auxiliary diagnosing and early diagnosing of NSCLC.