肺结核患者外周血中Treg细胞和Th17细胞等4种T细胞亚群与其发病的关系

Relation between 4 kinds of peripheral blood T cell subsets of Treg cell and Th17 cell in patients with pulmonary tuberculosis and its onset

目的通过检测肺结核患者和健康人群外周血中Treg细胞、Th17细胞以及CD8^+CD28^+T细胞和CD8^+CD28-T细胞的数量,探讨各细胞亚群的数量与肺结核发病的关系,为结核的诊断、治疗及预后提供新思路。方法应用流式细胞技术检测Treg细胞、Th17细胞以及CD8^+CD28^+T细胞和CD8^+CD28-T细胞,分别比较肺结核组、初治组、复治组与健康对照组之间的上述指标是否存在差异。结果 (1)肺结核组、初治组及复治组外周血中Th17细胞的数量分别为(0.74±0.33)%、(0.76±0.34)%、(0.73±0.32)%,CD8^+CD28^+T细胞的数量分别为(39.5±11.4)%、(39.5±10.9)%、(38.7±12.2)%,均分别低于健康对照组(1.17±0.38)%、(62.5±12.0)%,差异有统计学意义(P<0.01);(2)肺结核组、初治组及复治组外周血中Treg细胞的数量分别为(10.1±2.7)%、(11.4±3.8)%、(9.4±2.5)%,CD8^+CD28-T的数量分别为(60.5±11.4)%、(60.5±10.9)%、(61.3±12.2)%,均分别高于健康对照组的(7.2±2.2)%、(37.5±12.0)%,差异有统计学意义(P<0.01);(3)初治组患者与复治组患者外周血中Th17细胞、Treg细胞、CD8^+CD28^+T细胞、CD8^+CD28-T细胞数量之间的差异无统计学意义(P>0.05)。结论肺结核患者外周血中的效应性T细胞亚群Th17细胞和CD8^+CD28^+T细胞下降,具有抑制功能的CD4^+CD25^+CD127low/-Treg细胞和CD8^+CD28-T细胞升高,以上4个T细胞亚群可能与肺结核的发病过程相关。

Objective To investigate the relationship between the number of various cellular subsets and pulmonary tuberculosis（TB）onset by detecting the number of peripheral blood Treg cells,Th 17 cells,CD8^＋CD28^＋T cells and CD8^＋CD28^-T cells in the patients with pulmonary TB and healthy population so as to provide a new thinking for the diagnosis,treatment and prognosis of TB.Methods Treg cells,Th 17 cells,CD8^＋CD28^＋T cells and CD8^＋CD28^-T cells were detected by using flow cytometry（FCM）.The differences of these indexes were compared among the pulmonary TB group,initial treatment group,re-treatment group and healthy control group.Results（1）The percentages of peripheral blood Th17 cells and CD8^＋CD28^＋T cells in the TB group,initial treatment group and re-treatment group respectively were（0.74±0.33）%,（0.76±0.34）%,（0.73±0.32）% and（39.5±11.4）%,（39.5±10.9）%,（38.7±12.2）% respectivel;which were lower than（1.17±0.38）% and（62.5±12.0）%in the control group,the difference was statistical significance（P〈0.01）;（2）the percentages of peripheral blood Treg cells and CD8^＋CD28^-T cells in the TB group,initial treatment group and re-treatment group were（10.1±2.7）%,（11.4±3.8）%,（9.4±2.5）% and（60.5±11.4）%,（60.5±10.9）%,（61.3±12.2）% respectively;which were higher than（7.2±2.2）% and（37.5±12.0）%in the healthy control group,the difference was statistical significance（P〈0.01）;（3）the percentages of peripheral blood Th17 cells,Treg cells,CD8^＋CD28^＋T cells and CD8^＋CD28^-T cells had no statistical difference between the initial treatment group and re-treatment group（P〈0.05）.Conclusion The percentage of peripheral blood effect T cell subsets Th17 cells and CD8^＋CD28^-T cells in the patients with pulmonary TB is decreased,while the percentage of CD4^＋CD25^＋CD127^low/-Treg cells and CD8^＋CD28^-T cells with the inhibiting effect is increased,these 4kinds of T cell subsets may be related to the onset process of pulmonary TB.