葡萄抗炭疽病QTL的初步定位分析

Preliminary Analysis of Grape Anthracnose Resistance QTL

以欧亚种葡萄‘里扎马特’为母本,中国野生刺葡萄‘黑珍珠’为父本进行杂交,随机选择92株后代为作图群体,借助SSR和SRAP分子标记构建遗传图谱,采用区间作图法,对葡萄抗炭疽病相关QTL进行检测并分析。在12号连锁群上检测到1个抗炭疽病相关QTL,其可解释表型变异37.07%,贡献率为71.50%。这表明在12号连锁群检测到的QTL是抗葡萄炭疽病的1个主效QTL。

Ninety-two F1 individuals from the interspecific cross between‘Rizamat’（Vitis vinifera）and‘Heizhenzhu’（V. davidii）were used to construct a molecular genetic linkage map by using simple sequence repeats（SSR）and sequence-related amplified polymorphism（SRAP）. The interval mapping method was used to detect and analyze the grape anthracnose resistance QTL. A QTL was identified on linkage group 12，which explained 37.07% of the total phenotypic variance，and 71.50% contribution rate of the highest point. It showed that this QTL on linkage group 12 was a major QTL，which controlled the resistance to grape anthracnose.