苹果根皮苷糖基转移酶基因克隆及生物信息学分析

Clone and Bioinformatics Analysis of Phloridzin Glycosyltransferase from Malus x Domestica

糖基化反应是植物次级代谢物合成的重要反应。苹果根皮苷生物合成需要糖基转移酶的催化。本研究基于PCR的c DNA基因克隆技术对3个苹果根皮苷糖基转移酶进行克隆测序及生物信息学分析,结果表明,UGT71A15,UGT71K1和UGT88F1基因c DNA长度分别为1 416,1 434 bp和1 452 bp;其二级结构中含有较多的α-螺旋,占40%~46%;N端和C端均有典型的Rossmann折叠,含有PLN02992结构域;均具有亲水性,不含跨膜结构域,无信号肽,存在于叶绿体或细胞质中。本结果为进一步研究苹果根皮苷糖基转移酶的酶学性质及分离纯化提供理论依据。

Glycosylation is an important reaction in synthesis of plant secondary products. The last step in the biosynthesis of phloridzin needs to be catalyzed by glycosyltransferase. Using a PCR-based cloning strategy,three glycosyltransferase genes were cloned and their bioinformatics were analysed. It was shown that the c DNA clones for these glycosyltransferase were respectively 1 416, 1 434 and 1 452 nucletides in length; there were moreα-helix, accounting for40%-46%, and there were typically Rossmann folds in amino terminal and carboxy terminal, containing PLN02992 domain; glycosyltransferases were hydrophilic, existed in chloroplast or cytoplasm and had no transmembrane domain or signal peptide. This study provided theoretical basis for further discussion of enzymetic property, isolation and purification of apple phloridzin glycosyltransferase.