UVA诱导海参体壁相关酶活力的变化研究

Changes of Enzymes Activity in the Body Wall of Stichopus japonicus Induced by UVA irradiation

利用UVA(15 W/m2)照射活体海参体壁0 min,15 min,30 min,1 h,2 h和3 h,分别测定组织蛋白酶L(CL)、乙酰胆碱酯酶(ACh E)、超氧化物歧化酶(SOD)、过氧化氢酶(CAT)和谷胱甘肽过氧化物酶(GPx)的酶活力变化,从而探讨海参体壁自溶的机理。结果表明,在UVA照射1/2 h后,与0 h组相比,CL活力升高(24.09±5.47)%,ACh E,SOD,CAT和GPx活力分别降低(53.88±6.00)%,(33.43±2.27)%,(34.00±9.85)%和(19.78±11.00)%。UVA照射3 h,与0 h组相比,CL活力升高(97.75±1.71)%,ACh E,SOD,CAT和GPx活力分别降低(63.39±2.39)%,(53.71±1.99)%,(61.90±7.26%)和(24.86±0.01)%。本结果从酶活方面丰富了海参自溶的机理,为解决海参加工贮藏中由自溶引起的损失提供了方法。

To further explore the mechanism of sea cucumber bodywall, in this study, the living sea cucumber body wall was exposed to UVA irradiation（15 W/m2） for 0 min,15 min,30 min,1 h,2 h and 3 h, then detect changes of cathepsin L（CL）, acetylcholinesterase（ACh E）, superoxide dismutase（SOD）, catalase（CAT） and glutathione peroxidase（GPx） activity, respectively. The results showed that after exposure to 30 min, compared with the section exposed to UVA for 0 h, the activity of CL increased about（24.09 ± 5.47）%, and the activity of ACh E, SOD, CAT and GPx decreased（53.88 ± 6.00）%,（33.43 ± 2.27）%,（34.00 ± 9.85）% and（19.78 ± 11.00）%, respectively. After exposure for 3h, compared with the section exposed to UVA for 0 h, the activity of CL increased about（97.75 ± 1.71）%, and the activity of ACh E, SOD, CAT and GPx decreased 63.39%,（53.71 ± 1.99）%,（61.90 ± 7.26）% and（24.86 ± 0.01）%, respectively. The above results enrich the theory and advance our understanding of the mechanism of autolysis of sea cucumber.