摘要
Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog （Scutiger boulengeri）.To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from will offer useful tools in detecting population structure Tibetan Plateau, where the species resides. the Hardy-Weinberg equilibrium. We hope that these markers of S. boulengeri and in monitoring the fragile ecosystem of the
Fifteen microsatellite DNA markers were developed from a transcriptome assembly of the alpine stream frog （Scutiger boulengeri）.To characterize these loci, we genotyped 23 individuals collected from two sites. Eleven loci were variable, with the number of alleles ranging from one to six within each population. The expected and observed heterozygosities ranged from 0 to 0.78 and from 0 to 0.64, respectively. None of the loci was in linkage disequilibrium and one locus for one population was deviated from will offer useful tools in detecting population structure Tibetan Plateau, where the species resides. the Hardy-Weinberg equilibrium. We hope that these markers of S. boulengeri and in monitoring the fragile ecosystem of the
基金
supported by a scholarship from the China Scholarship Council to Z.DU
