通络醒脑泡腾片中4种成分含量的RP-HPLC法同时测定

Simultaneous Determination of 4 Ingredients in Tongluoxingnao Effervescent Tablets by RP-HPLC

目的 建立通络醒脑泡腾片中4种成分的含量测定方法。方法 采用反相高效液相法（RP-HPLC）,分析柱：Wondasil C18-WR色谱柱（4.6mm×250mm,5μm）,以甲醇-0.5%磷酸水为流动相进行梯度洗脱,流速控制在1.0ml/min,检测波长为280nm,柱温35℃。结果 阿魏酸、黄芩苷、汉黄芩苷、藁本内酯线性范围分别为1.79~16.10ng、95.30~857.66ng、18.31~164.76ng、13.52~121.65ng;加样回收率分别为93.07%（RSD=1.18%）、101.64%（RSD=0.89%）、100.32%（RSD=0.98%）、95.59%（RSD=2.25%）。结论 所建立的方法简便,准确,快速,可用于通络醒脑泡腾片的质量控制。

Objective To develop an HPLC method for the determination of 4 ingredients in Tongluoxingnao effervescent tablets. Methods The sample was separated on Wondasil C18 -WR （250mm × 4.6mm , 5μm） ; with CH3OH-0.5% Phosphoric acid solution by gradient elution; flow rate： 1.0ml/min; UV detection wavelength was at 280nm; column temperature was at 35℃. Results The concentration of Ferulic acid, Baiealin, Wogonoside, Ligustilide in Tongluoxingnao effervescent tablets within 1.79 ~ 16.10ng,95.30 ~ 857.66ng, 18.31 ~ 164.76ng, 13.52 ~ 121.65ng were in a good linear relationship, the average recovery was 93.07 % （RSD = 1.18 % ）, 101.64% （RSD = 0.89% ）, 100.32 % （RSD = 0.98 % ）, 95.59% （RSD = 2.25 % ）. Conclusion It was a simple, sensitive and good repeatability method and could be used to control the quality of Tongluoxingnao effervescent tab- lets.