β-Casein-15肽在抗新生儿常见致病菌中的作用

β-Casein-15 peptide role in neonatal common pathogenic bacteria resistance

目的研究乳源性多肽对新生儿抗感染作用,为生命早期健康成长提供营养保障。方法利用质谱定量技术比较β-Casein-15肽在早产和足月产产妇母乳中的含量差异;通过Uniprot、SABLE、ProtParam tool在线数据库预测β-Casein-15肽生物学特征;以抑菌圈实验以及免疫荧光实验来检测β-Casein-15肽对大肠杆菌、小肠结肠炎菌、金黄色葡萄球菌的抑菌作用。结果通过质谱分析以及生物信息学分析发现:β-Casein-15肽的脂肪指数是122.33,亲水性是-0.387,不稳定系数是36.29。这些数据表明β-Casein-15肽是稳定性疏水性多肽。质谱定量分析发现β-Casein-15肽在早产儿产妇母乳中含量明显高于足月儿产妇,抑菌实验发现β-Casein-15肽对大肠杆菌、小肠结肠炎菌以及金黄色葡萄球菌生长均有明显的抑制作用。结论这些结果提示β-Casein-15肽在抗新生儿感染中发挥着重要的作用。

Objective To study the human milk resource peptide in the role of resistance of neonatal infection. Methods The content of β-Casein-15 in human milk was mesured by mass spectrometry technology. The biologic characteristics of β-Casein-15 peptide were predicted by Uniprot,SABLE,ProtParam tool and other online databases. Results The online databases showed that aliphatic index, grand average of hydropathicity and instability coefficient of β-Casein-15 were 122. 33,-0. 387 and 36.29, respectively. It indicated that β-Casein-15 was a hydrophobic stable peptide. And then, the content of β-Casein-15 was significantly decreased in premature compared to full term infants,detected by mass spectrometry technology. Moreover, the bacteriostasis experiment was found β-Casein-15 peptide in e. coli, enterocolitis bacteria and staphylococcus aureus had obvious bacteriostatic action. Conclusion These results indicate β-Casein-15 may play a critial role in promoting neonatal anti-infection.