低剂量鱼藤酮诱导BV2细胞IL-6产物及montelukast的作用

Low-dose rotenone-induced interleukin-6 production and the effect of montelukast in BV2 cells

目的在体外帕金森病（Parkinson＇s disease,PD）模型观察低剂量鱼藤酮诱导的BV2小胶质细胞白介素6（IL-6）生成及montelukast的作用。方法以PD诱导因子鱼藤酮处理小鼠小胶质细胞系BV2细胞。Western blot方法检测BV2细胞前炎症细胞因子IL-6蛋白表达,ELISA方法检测BV2细胞IL-6释放。观察Cys LT1R拮抗剂montelukast对鱼藤酮诱导的小胶质细胞IL-6的影响。结果低剂量鱼藤酮（0.3～3 n M）浓度依赖性地增加IL-6蛋白表达;以鱼藤酮（3 n M）处理细胞1～24h,时间依赖性的诱导IL-6释放增加。Cys LT1R拮抗剂montelukast降低鱼藤酮增高的BV2细胞IL-6水平。结论低剂量鱼藤酮诱导小胶质细胞前炎症细胞因子IL-6增加,montelukast抑制鱼藤酮诱导BV2细胞效应。

Objective To understand low-dose rotenone-induced interleukin-6（ IL-6） production and the effect of montelukast in an in vitro model of Parkinson＇s disease（ PD）. Method BV-2 cells,an immortalized cell line of murine microglial cells,were treated with PD inducer rotenone. The expression of proinflammatory cytokine（ IL-6） protein was detected with Western blot,and the level of IL-6 in the supernatants was analyzed by ELISA. The effect of Cys LT1 R antagonist montelukast on rotenone-induced microglial IL-6 production was investigated. Findings Treatment with low-dose rotenone（ 0. 3- 3 n M） dose-dependently increased cytokine IL-6 expression in BV2 cells. Exposure to（ 3 n M） rotenone for 1- 24 h induced IL-6 release from BV2 cells in a time-dependent manner. Cys LT1 R antagonist montelukast（ 0. 1 and 1μM） significantly reduced IL-6 production in rotenone（ 3 n M）-treated BV2 cells. Conclusion Low-dose rotenone can induce microglial proinflammatory cytokine IL-6 production. Montelukast can effectively inhibit low-dose rotenone-induced microglial response.