荧光PCR检测手足口病病毒结果分析

Analysis of the detection results of hand,foot and mouth virus with fluorescent PCR

目的了解本地区手足口病的病原体及病毒基因分型,为制定预防和控制肠道病毒感染提供依据。方法采用实时荧光定量PCR方法,用人肠道病毒通用型引物、肠道病毒71型引物、柯萨奇病毒16型引物和柯萨奇病毒A6型引物,对480份手足口病标本进行特异性核酸检测。结果 480份手足口病标本经PCR扩增后,检出阳性标本253份,其中EV71型94份,Cox A16型137份,A6型22份,检出率分别为19.6%、28.5%、4.6%。3岁~4岁儿童发病率最高,为26%;其次为4岁~5岁儿童,发病率为20%。结论本地区手足口病以Cox A16型为主,3岁~5岁儿童发病率较高。

Objective To investigate the pathogens and virus genotyping of hand,foot and mouth disease（ HFMD）,so as to provide basis for the prevention and control of intestinal virus infection. Methods Human enterovirus universal primers and enterovirus 71 primers,Coxsackie virus type 16 primers and coxsackievirus A6 primers were used for the specific detection of nucleic acid in 480 HFMD by real time fluorescence quantitative PCR. Results After amplification by PCR,253 were positive in the 480 specimens,including 94 cases of EV71,137 cases of Cox A16,22 cases of A6,respectively,with the positive rates of37. 2%,54. 2% and 8. 7%. Conclusion Cox A16 is dominant among 5-6 years old children infected with HFMD in this area.