当归补血药对对血虚便秘模型大鼠的治疗作用

Therapeutic effects of Angelicae sinensis herbal pairs on blood-deficiency( 血虚) constipation in rats

目的:观察当归补血药对对血虚便秘模型大鼠的治疗作用。方法:用乙酰苯肼、环磷酰胺和洛哌丁胺联合复制血虚便秘大鼠模型,观察当归补血药对(当归-黄芪、当归-白芍、当归-熟地黄)对血虚便秘大鼠体征、体重、外周血象、排便情况、肠道传输时间和结肠组织形态的影响,研究当归补血药对的补血润肠作用;ELISA法测定大鼠血清胆囊收缩素(CCK-8)、β-内啡肽(β-EP)、胃动素(MOT)和生长抑素(SS)的含量变化,研究当归补血药对对血虚便秘模型大鼠胃肠激素的影响;从外周血象和血清胃肠激素水平两方面用偏最小二乘判别分析法(PLS-DA)综合分析当归补血药对对模型大鼠的治疗规律,初步比较当归补血药对润肠通便的差异。结果:单用当归及当归补血药对(6g/kg)均能不同程度改善模型大鼠便秘症状和血虚体征,使大鼠12h排便量和粪便含水量显著增加;与模型对照组比较,单用当归、当归-黄芪和当归-熟地黄均能显著缩短首粒黑便排出时间,均显著升高模型大鼠白细胞(WBC)、红细胞(RBC)、血红蛋白(HGB)和红细胞压积(HCT);单用当归的大鼠血清CCK-8、MOT含量显著升高、血清β-EP含量显著降低,当归-黄芪和当归-熟地黄组大鼠血清MOT含量显著升高,血清β-EP、SS含量显著降低;当归-白芍组大鼠血清β-EP、SS含量显著降低;PLS-DA综合分析显示,WBC、HGB和血清CCK-8是研究当归补血药对润肠通便效应的关键指标,当归-黄芪、当归-熟地黄药对具有较强的作用优势。结论:当归及当归补血药对对血虚便秘模型大鼠具有确定的治疗作用,其作用机制可能与调节外周血象和体内CCK-8、MOT、β-EP、SS的含量有关,当归-黄芪、当归-熟地黄药对的效应较强。

Objective： To observe the therapeutic effects of Angelicae Sinensis herbal pairs on blood-deficiency constipation in rats. Methods：Acetylphenyhydrazine,cyclophosphamide and loperamide were used to copy the blood-deficiency constipation model in rats. By observing effects of Angelicae Sinensis radix herb pairs（ Angelicae sinensis-Astragali Radix（ Danggui-Huangqi）,Angelicae sinensis-Paeoniae Alba（ Danggui-Baishao）,Angelicae sinensis-Rehmanniae preparata（ Danggui-Dihuang）） on general physical signs,body weights,periphera hemogram,defecation situation,gastrointestinal motility and colon tissue appearance in rats to study the effects in smoothing intestine and relieving constipation. The level of Chylecystokinin-8（ CCK-8）,β-endorphin（ β-EP）,Gastrin（ MOT） and Somatostatin（ SS） in blood were measured by enzyme-linked immunosorbent（ ELISA） method,to explore its possible mechanism. Partial least-squares discriminant analysis（ PLSDA） was used to analyze different treatment rules in peripheral hemogram and serum gastrointestinal hormone levels. Results： The symptoms of blood-deficiency constipation in rats can be improved by Angelicae Sinensis radix herb pairs（ 6g/kg）,body weight,total number of defecation grains in 12 h and stool water content were increased（ P〈0. 05,P〈0. 01）. Compared with model control group,the time of the first black faeces were shortened significantly in Danggui,Danggui-Huangqi and Danggui-Dihuang groups（ P〈0. 05,P〈0. 01）. In addition to the level of RBC in Danggui-Baishao,Danggui,Danggui-Huangqi,Danggui-Baishao and Danggui-Dihuang could increased the levels of WBC,RBC,HGB,HCT（ P〈0. 05,P〈0. 01） in blood. The levels of CCK-8,MOT were higher and the levels of β-EP were lower（ P〈0. 05,P〈0. 01） in Danggui group. The levels of MOT were higher and the levels of β-EP,SS in blood were lower（ P〈0. 05,P〈0. 01） in Danggui-Huangqi and Shudihuang group. The levels of β-EP,SS in blood were lower（ P〈0. 05,P〈0. 01） in Danggui-Baishao group. PLS-DA analysis showed that the effection of enriching blood and embellish aperient bowel of Angelicae Sinensis radix herb pairs were focus on the levels of WBC and HGB in rats,the mechanism were focus on the levels of CCK-8 in serum. Conclusion： Danggui,Danggui-Huangqi and Danggui-Dihuang have certain therapeutic effects on blood deficiency constipation in rats; the mechanism of action may be related to the contents of CCK-8,MOT,β-EP,SS and all of cells in blood.