富含半胱氨酸分泌性酸性蛋白在乳腺癌组织中的表达及其临床意义

Clinical significance of secreted protein acidic and rich in cysteine expression in breast cancer tissue

目的观察富含半胱氨酸分泌性酸性蛋白(SPARC)在人乳腺癌组织中的表达及其与患者临床病理特征和预后的关系。方法选取122例乳腺癌患者的完整肿瘤组织蜡块为研究对象,用免疫组化法检测癌组织中SPARC的表达水平,并分析其与临床病理特征及预后的关系。结果 SPARC主要表达于乳腺癌组织的细胞质和细胞间质,并弥漫分布,呈现深棕紫色颗粒状。SPARC在乳腺癌组织中的阳性表达率为72.13%(88/122例)。SPARC在乳腺组织中的阳性表达与患者的年龄、月经状况、TNM分期、肿瘤大小、孕激素水平、人类表皮生长因子受体2(HER-2)无明显相关性(P>0.05),与肿瘤分化程度、雌激素受体水平及淋巴结转移存在明显相关性(P<0.05)。SPARC阳性和阴性组3年无疾病进展生存率分别为69.92%和86.13%,差异有统计学意义(P<0.05)。结论 SPARC与乳腺癌的发生发展密切相关,且与雌激素受体水平及淋巴结转移有关,可作为乳腺癌复发的肿瘤标志物。

Objective To discuss the secreted protein acidic and rich in cysteine（ SPARC） expression in breast cancer tissue and its associationwith clinical pathology and prognosis. Methods One hundred and twenty-two cases of breast cancer who underwent surgery form May 2009 to June 2015 were included in this study. The SPARC expression in cancer tissue was tested by immunohistochemical method. The expression status,clinical pathology characteristics and prognosis were evaluated. Results SPARC protein was expressed in the cytoplasm and cytoplasm of tumor tissues with dark brown and purple stain. The positive rate were 72. 13%（ 88 /122 cases） in cancer tissue. The positive expression of SPARC in breast tissues was not significantly correlated with age,menstruation status,TNM stage,tumor size,progesterone level,human epidermal growth factor receptor-2（ HER-2） status（ P〉0. 05）. But there was a correlation between tumor differentiation,estrogen receptor and lymph node metastasis（ P 〈0. 05）. The 3-year progression free survival rates were 86. 13% and 69. 92% in SPARC negative and positive groups with statistically significant difference（ P〈0. 05）. Conclusion SPARC is closely related to the development of breast cancer,which can be used as a tumor marker for breast cancer recurrence.